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CEBPD elisa kit :: Human CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS766081
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta), ELISA Kit

 Also Known As   

Human CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta)

 Product Synonym Names    CELF/CRP3; C/EBP-delta/NF-IL6-beta
 Product Gene Name   

CEBPD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    116898
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CEBPD . No significant cross-reactivity or interference between CEBPD and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    31.2-2000pg/ml
 Sensitivity    18.75pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CEBPDwere tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CEBPDwere tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    4 degree C for 6 months
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CEBPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CEBPD purchase    MBS766081 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CEBPD (CCAAT/enhancer Binding Protein (C/EBP) delta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000pg/ml in biological research samples containing CEBPD, with an estimated sensitivity of 18.75pg/ml. The ELISA analytical biochemical technique of the MBS766081 kit is based on CEBPD antibody-CEBPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CEBPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CEBPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CEBPD elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CEBPDantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CEBPD antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CEBPD amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CEBPD can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of CEBPD elisa kit    CEBPD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS766081. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CEBPD. It may not necessarily be applicable to this product.
 NCBI GI #    28872798
 NCBI GeneID    1052
 NCBI Accession #    NP_005186.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005195.3 [Other Products]
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 UniProt Secondary Accession #    Q14937; Q2M2X9 [Other Products]
 UniProt Related Accession #    P49716 [Other Products]
 Molecular Weight    28,467 Da
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 NCBI Official Full Name    CCAAT/enhancer-binding protein delta
 NCBI Official Synonym Full Names    CCAAT/enhancer binding protein delta
 NCBI Official Symbol    CEBPD [Similar Products]
 NCBI Official Synonym Symbols   
CELF; CRP3; C/EBP-delta; NF-IL6-beta
[Similar Products]
 NCBI Protein Information    CCAAT/enhancer-binding protein delta
 UniProt Protein Name    CCAAT/enhancer-binding protein delta
 UniProt Synonym Protein Names   
Nuclear factor NF-IL6-beta
 Protein Family    CCAAT/enhancer-binding protein
 UniProt Gene Name    CEBPD [Similar Products]
 UniProt Synonym Gene Names    NF-IL6-beta [Similar Products]
 UniProt Entry Name    CEBPD_HUMAN
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 NCBI Summary for CEBPD    The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11. [provided by RefSeq, Sep 2010]
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 UniProt Comments for CEBPD    C/EBP-delta: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha. Interacts with SPI1/PU.1. Interacts with PRDM16. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 8p11.2-p11.1

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
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 Research Articles on CEBPD    1. We have discovered the CEBPD-hypoxia-inducible factor-1 (HIF-1) pathway as a new HIF-1-regulating fundamental mechanism in tubular epithelial cells for the cellular protection against kidney injury.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CEBPD elisa kitDiseases associated with CEBPD elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 IL17 Signaling Pathway antibodies  IL17 Signaling Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
 Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies  Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >45 publications with CEBPD and Inflammation
 Necrosis Antibodies  >20 publications with CEBPD and Necrosis
 Nervous System Diseases Antibodies  >19 publications with CEBPD and Nervous System Diseases
 Breast Neoplasms Antibodies  >13 publications with CEBPD and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >11 publications with CEBPD and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >11 publications with CEBPD and Disease Models, Animal
 Liver Diseases Antibodies  >10 publications with CEBPD and Liver Diseases
 Lung Diseases Antibodies  >8 publications with CEBPD and Lung Diseases
 Adenocarcinoma Antibodies  >8 publications with CEBPD and Adenocarcinoma
 Liver Neoplasms Antibodies  >8 publications with CEBPD and Liver Neoplasms
Organs/Tissues associated with CEBPD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >36 publications with CEBPD and Muscle
 Liver Antibodies  >26 publications with CEBPD and Liver
 Vascular Antibodies  >24 publications with CEBPD and Vascular
 Bone Antibodies  >23 publications with CEBPD and Bone
 Lung Antibodies  >20 publications with CEBPD and Lung
 Brain Antibodies  >19 publications with CEBPD and Brain
 Heart Antibodies  >18 publications with CEBPD and Heart
 Bone Marrow Antibodies  >12 publications with CEBPD and Bone Marrow
 Connective Tissue Antibodies  >11 publications with CEBPD and Connective Tissue
 Mammary Gland Antibodies  >8 publications with CEBPD and Mammary Gland
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