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CENPE elisa kit :: Human CENPE ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502176
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CENPE, ELISA Kit

★Popular Item★
 Also Known As   

Human CENPE (Centromere Protein E) ELISA Kit

 Product Gene Name   

CENPE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    117143
 3D Structure    ModBase 3D Structure for Q02224
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanCENPE. No significant cross-reactivity or interference between HumanCENPE and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CENPE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CENPE purchase    MBS2502176 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CENPE, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing CENPE, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2502176 kit is based on CENPE antibody-CENPE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CENPE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CENPE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CENPE elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCENPEconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CENPE. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CENPEand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CENPE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CENPE.You can calculate the concentration of CENPEin the samples by comparing the OD of the samples to the standard curve. FOR
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 Typical Testing Data/Standard Curve (for reference only) of CENPE elisa kit    CENPE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502176. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CENPE. It may not necessarily be applicable to this product.
 NCBI GI #    557878612
 NCBI GeneID    1062
 NCBI Accession #    NP_001273663.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001286734.1 [Other Products]
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 UniProt Primary Accession #    Q02224 [Other Products]
 UniProt Secondary Accession #    Q4LE75; A6NKY9; A8K2U7 [Other Products]
 UniProt Related Accession #    Q02224 [Other Products]
 Molecular Weight    301,789 Da
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 NCBI Official Full Name    centromere-associated protein E isoform 2 proprotein
 NCBI Official Synonym Full Names    centromere protein E, 312kDa
 NCBI Official Symbol    CENPE [Similar Products]
 NCBI Official Synonym Symbols   
KIF10; CENP-E; MCPH13; PPP1R61
[Similar Products]
 NCBI Protein Information    centromere-associated protein E; Centromere autoantigen E (312kD); kinesin family member 10; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
 UniProt Protein Name    Centromere-associated protein E
 UniProt Synonym Protein Names   
Centromere protein E; CENP-E; Kinesin-related protein CENPE
 Protein Family    Centromere-associated protein
 UniProt Gene Name    CENPE [Similar Products]
 UniProt Synonym Gene Names    CENP-E [Similar Products]
 UniProt Entry Name    CENPE_HUMAN
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 NCBI Summary for CENPE    Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]
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 UniProt Comments for CENPE    CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: kinetochore; microtubule cytoskeleton; kinesin complex; microtubule; membrane; cytoplasm; outer kinetochore of condensed chromosome; midbody; chromosome; cytosol; nucleus; chromosome, pericentric region

Molecular Function: kinetochore binding; protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: kinetochore assembly; attachment of spindle microtubules to kinetochore during mitosis; metabolic process; cell division; positive regulation of protein kinase activity; multicellular organismal development; regulation of mitotic metaphase/anaphase transition; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; mitotic chromosome movement towards spindle pole; blood coagulation; mitotic metaphase plate congression; microtubule-based movement; chromosome segregation

Disease: Microcephaly 13, Primary, Autosomal Recessive
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 Research Articles on CENPE    1. Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CENPE elisa kitPathways associated with CENPE elisa kit
 Reference Product  PubMed Publications
 AURKB elisa kit  >5 publications with CENPE and AURKB
 CENPF elisa kit  >5 publications with CENPE and CENPF
 BUB1B elisa kit  >4 publications with CENPE and BUB1B
 CDC20 elisa kit  >2 publications with CENPE and CDC20
 CENPA elisa kit  >1 publications with CENPE and CENPA
 CDCA8 elisa kit  >1 publications with CENPE and CDCA8
 PLK1 elisa kit  >1 publications with CENPE and PLK1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Kinesins Pathway antibodies  Kinesins Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 MHC Class II Antigen Presentation Pathway antibodies  MHC Class II Antigen Presentation Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
Diseases associated with CENPE elisa kitOrgans/Tissues associated with CENPE elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4 publications with CENPE and Breast Neoplasms
 Adenoma Antibodies  >3 publications with CENPE and Adenoma
 Neoplasms, Experimental Antibodies  >2 publications with CENPE and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >2 publications with CENPE and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >2 publications with CENPE and Lung Neoplasms
 Disease Models, Animal Antibodies  >1 publications with CENPE and Disease Models, Animal
 Pancreatic Neoplasms Antibodies  >1 publications with CENPE and Pancreatic Neoplasms
 Skin Neoplasms Antibodies  >1 publications with CENPE and Skin Neoplasms
 Fetal Growth Retardation Antibodies  >1 publications with CENPE and Fetal Growth Retardation
 Fibrosis Antibodies  >1 publications with CENPE and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with CENPE and Lung
 Skin Antibodies  >2 publications with CENPE and Skin
 Brain Antibodies  >2 publications with CENPE and Brain
 Liver Antibodies  >1 publications with CENPE and Liver
 Connective Tissue Antibodies  >1 publications with CENPE and Connective Tissue
 Kidney Antibodies  >1 publications with CENPE and Kidney
 Embryonic Tissue Antibodies  >1 publications with CENPE and Embryonic Tissue
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