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CFL1 elisa kit :: Human CFL1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505977
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CFL1, ELISA Kit

★Popular Item★
 Also Known As   

Human CFL1(Cofilin 1, Non-Muscle) ELISA Kit

 Product Gene Name   

CFL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601442
 3D Structure    ModBase 3D Structure for P23528
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human CFL1. No significant cross-reactivity or interference between Human CFL1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CFL1 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156--10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CFL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFL1 purchase    MBS2505977 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CFL1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156--10ng/mL in biological research samples containing CFL1, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2505977 kit is based on CFL1 antibody-CFL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CFL1 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFL1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFL1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CFL1. You can calculate the concentration of Human CFL1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CFL1 elisa kit    CFL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505977. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CFL1. It may not necessarily be applicable to this product.
 NCBI GI #    1177471
 NCBI GeneID    1072
 NCBI Accession #    CAA64685.1 [Other Products]
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 UniProt Primary Accession #    P23528 [Other Products]
 UniProt Secondary Accession #    Q53Y87; Q9UCA2; B3KUQ1 [Other Products]
 UniProt Related Accession #    P23528 [Other Products]
 Molecular Weight    18,502 Da
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 NCBI Official Full Name    cofilin
 NCBI Official Synonym Full Names    cofilin 1 (non-muscle)
 NCBI Official Symbol    CFL1 [Similar Products]
 NCBI Official Synonym Symbols   
CFL; HEL-S-15
[Similar Products]
 NCBI Protein Information    cofilin-1; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15; p18
 UniProt Protein Name    Cofilin-1
 UniProt Synonym Protein Names   
18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
 Protein Family    Cofilin
 UniProt Gene Name    CFL1 [Similar Products]
 UniProt Synonym Gene Names    CFL; p18 [Similar Products]
 UniProt Entry Name    COF1_HUMAN
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 NCBI Summary for CFL1    The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
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 UniProt Comments for CFL1    Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: axon guidance; platelet activation; actin filament depolymerization; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis
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 Research Articles on CFL1    1. It provides a brief overview of the current knowledge about the central role of cofilin/actin rods formation, where its dysregulation and malfunction might be the trigger to neurodegeneration.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CFL1 elisa kitPathways associated with CFL1 elisa kit
 Reference Product  PubMed Publications
 LIMK1 elisa kit  >17 publications with CFL1 and LIMK1
 SSH1 elisa kit  >7 publications with CFL1 and SSH1
 PFN1 elisa kit  >4 publications with CFL1 and PFN1
 ACTG1 elisa kit  >2 publications with CFL1 and ACTG1
 ACTB elisa kit  >2 publications with CFL1 and ACTB
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
Diseases associated with CFL1 elisa kitOrgans/Tissues associated with CFL1 elisa kit
 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >10 publications with CFL1 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >9 publications with CFL1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >7 publications with CFL1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >7 publications with CFL1 and Breast Neoplasms
 Inflammation Antibodies  >5 publications with CFL1 and Inflammation
 Neoplasms, Experimental Antibodies  >4 publications with CFL1 and Neoplasms, Experimental
 Neoplasm Invasiveness Antibodies  >3 publications with CFL1 and Neoplasm Invasiveness
 Cardiovascular Diseases Antibodies  >2 publications with CFL1 and Cardiovascular Diseases
 Memory Disorders Antibodies  >2 publications with CFL1 and Memory Disorders
 Kidney Diseases Antibodies  >1 publications with CFL1 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >18 publications with CFL1 and Brain
 Muscle Antibodies  >14 publications with CFL1 and Muscle
 Blood Antibodies  >12 publications with CFL1 and Blood
 Nerve Antibodies  >11 publications with CFL1 and Nerve
 Lung Antibodies  >9 publications with CFL1 and Lung
 Kidney Antibodies  >6 publications with CFL1 and Kidney
 Uterus Antibodies  >5 publications with CFL1 and Uterus
 Vascular Antibodies  >4 publications with CFL1 and Vascular
 Eye Antibodies  >3 publications with CFL1 and Eye
 Embryonic Tissue Antibodies  >3 publications with CFL1 and Embryonic Tissue
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