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COL1alpha1 elisa kit :: Human COL1alpha1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2508669
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

COL1alpha1, ELISA Kit

★Popular Item★
 Also Known As   

Human COL1alpha1 (Collagen Type I Alpha 1) ELISA Kit

 Product Gene Name   

COL1alpha1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P05539
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human COL1alpha1. No significant cross-reactivity or interference between Human COL1alpha1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human COL1alpha1 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313--20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL1alpha1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL1alpha1 purchase    MBS2508669 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COL1alpha1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313--20ng/mL in biological research samples containing COL1alpha1, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2508669 kit is based on COL1alpha1 antibody-COL1alpha1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL1alpha1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL1alpha1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL1alpha1 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1alpha1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1alpha1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human COL1alpha1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1alpha1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human COL1alpha1. You can calculate the concentration of Human COL1alpha1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of COL1alpha1 elisa kit    COL1alpha1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2508669. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL1alpha1. It may not necessarily be applicable to this product.
 NCBI GI #    6978677
 NCBI GeneID    25412
 NCBI Accession #    NP_037061.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012929.1 [Other Products]
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 UniProt Primary Accession #    P05539 [Other Products]
 UniProt Secondary Accession #    Q63123; Q63565; Q78DY3 [Other Products]
 UniProt Related Accession #    P05539 [Other Products]
 Molecular Weight    134,570 Da
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 NCBI Official Full Name    collagen alpha-1(II) chain
 NCBI Official Synonym Full Names    collagen, type II, alpha 1
 NCBI Official Symbol    Col2a1 [Similar Products]
 NCBI Official Synonym Symbols   
CG2A1A; COLLII
[Similar Products]
 NCBI Protein Information    collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; collagen type II (Col2A1) gene, enhancer region; procollagen, type II, alpha 1
 UniProt Protein Name    Collagen alpha-1(II) chain
 UniProt Synonym Protein Names   
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
 UniProt Gene Name    Col2a1 [Similar Products]
 UniProt Entry Name    CO2A1_RAT
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 NCBI Summary for COL1alpha1    structural component of the cartilage extracellular matrix [RGD, Feb 2006]
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 UniProt Comments for COL1alpha1    Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
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 Research Articles on COL1alpha1    1. Rat collagen II citrullination decreased the adhesion of human synovial fibroblasts and rat mesenchymal stem cells by decreasing the binding of integrins alpha10beta1 and alpha11beta1 (but not alpha1beta1 and alpha2beta1)to Arg-containing motifs. It decreased MSC survival.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with COL1alpha1 elisa kitDiseases associated with COL1alpha1 elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Disease Name  Pubmed Publications
 Arthritis Antibodies  >235 publications with COL1alpha1 and Arthritis
 Osteoarthritis Antibodies  >233 publications with COL1alpha1 and Osteoarthritis
 Osteochondrodysplasias Antibodies  >145 publications with COL1alpha1 and Osteochondrodysplasias
 Retinal Detachment Antibodies  >55 publications with COL1alpha1 and Retinal Detachment
 Myopia Antibodies  >45 publications with COL1alpha1 and Myopia
 Cleft Palate Antibodies  >41 publications with COL1alpha1 and Cleft Palate
 Craniofacial Abnormalities Antibodies  >38 publications with COL1alpha1 and Craniofacial Abnormalities
 Necrosis Antibodies  >34 publications with COL1alpha1 and Necrosis
 Kniest dysplasia Antibodies  >27 publications with COL1alpha1 and Kniest dysplasia
 Hearing Loss, Sensorineural Antibodies  >24 publications with COL1alpha1 and Hearing Loss, Sensorineural
Organs/Tissues associated with COL1alpha1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >293 publications with COL1alpha1 and Connective Tissue
 Embryonic Tissue Antibodies  >105 publications with COL1alpha1 and Embryonic Tissue
 Brain Antibodies  >20 publications with COL1alpha1 and Brain
 Kidney Antibodies  >16 publications with COL1alpha1 and Kidney
 Heart Antibodies  >12 publications with COL1alpha1 and Heart
 Inner Ear Antibodies  >9 publications with COL1alpha1 and Inner Ear
 Lung Antibodies  >6 publications with COL1alpha1 and Lung
 Uncharacterized Tissue Antibodies  >5 publications with COL1alpha1 and Uncharacterized Tissue
 Spleen Antibodies  >1 publications with COL1alpha1 and Spleen
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