Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CNX rightarrow Human CNX  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • TK1 Recombinant Protein
 • Somatostatin (SST) Antibody
 • Arg2 Antibody
 • LMO4 Antibody
 • COX4I1 Antibody
 • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase,... Antibody
 • TARC ELISA Kit
 • Islet cell antibody (ICA) ELISA Kit
 • A Disintegrin and Metalloproteinase With Thrombospondin 1 (A... ELISA Kit
 • Varicella-Zoster Virus (Mixed) (VZV) Antibody
 • A Disintegrin And Metalloproteinase With Thrombospondin 1 (A... Antibody
 • SCFR ELISA Kit
 • Adenovirus Native Protein
 • Chlamydia IgM ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CNX elisa kit :: Human Calnexin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721668
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Calnexin (CNX), ELISA Kit

★Popular Item★
 Also Known As   

Human Calnexin ELISA Kit

 Product Gene Name   

CNX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    114217
Section Bar
 Species Reactivity    Human
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    1.0ng/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CNX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CNX purchase    MBS721668 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calnexin (CNX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing CNX, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS721668 kit is based on CNX antibody-CNX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CNX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CNX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for CNX elisa kit

   For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Uses: This CNX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CNX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CNX ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CNX. Standards or samples are then added to the microtiter plate wells and CNX if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CNX present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CNX are added to each well to "sandwich" the CNX immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CNX and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNX concentration in each sample is interpolated from this standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of CNX elisa kit    CNX elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS721668. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CNX. It may not necessarily be applicable to this product.
 NCBI GI #    66933005
 NCBI GeneID    821
 NCBI Accession #    NP_001019820.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001024649.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    B2R5V8; B4DGP8; B4E2T8; D3DWQ3; D6R9K3 [Other Products]
 UniProt Related Accession #    P27824 [Other Products]
 Molecular Weight    55,598 Da
Table BarTOPTable Bar
 NCBI Official Full Name    calnexin
 NCBI Official Synonym Full Names    calnexin
 NCBI Official Symbol    CANX [Similar Products]
 NCBI Official Synonym Symbols   
CNX; P90; IP90
[Similar Products]
 NCBI Protein Information    calnexin; major histocompatibility complex class I antigen-binding protein p88
 UniProt Protein Name    Calnexin
 UniProt Synonym Protein Names   
IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
 Protein Family    Calnexin
 UniProt Gene Name    CANX [Similar Products]
 UniProt Entry Name    CALX_HUMAN
Section Bar
 NCBI Summary for CNX    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CNX    Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum .Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: dendrite cytoplasm; endoplasmic reticulum membrane; smooth endoplasmic reticulum; protein complex; rough endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; dendritic spine; membrane; cell soma; axon; melanosome; ribosome

Molecular Function: ionotropic glutamate receptor binding; protein binding; unfolded protein binding; apolipoprotein binding; calcium ion binding; carbohydrate binding; glycoprotein binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; synaptic vesicle endocytosis; protein secretion; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging
Table BarTOPTable Bar
 Research Articles on CNX    1. The impact of CANX genetic variants to heart rate was discovered.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with CNX elisa kitPathways associated with CNX elisa kit
 Reference Product  PubMed Publications
 CALR elisa kit  >2 publications with CNX and CALR
 PDIA3 elisa kit  >1 publications with CNX and PDIA3
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Assembly Of Viral Components At The Budding Site Pathway antibodies  Assembly Of Viral Components At The Budding Site Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
Diseases associated with CNX elisa kitOrgans/Tissues associated with CNX elisa kit
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >2 publications with CNX and Adenocarcinoma
 Gastrointestinal Diseases Antibodies  >1 publications with CNX and Gastrointestinal Diseases
 Nervous System Diseases Antibodies  >1 publications with CNX and Nervous System Diseases
 Lung Diseases Antibodies  >1 publications with CNX and Lung Diseases
 Liver Diseases Antibodies  >1 publications with CNX and Liver Diseases
 Heart Diseases Antibodies  >1 publications with CNX and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with CNX and Lung
 Liver Antibodies  >2 publications with CNX and Liver
 Testis Antibodies  >2 publications with CNX and Testis
 Heart Antibodies  >1 publications with CNX and Heart
 Blood Antibodies  >1 publications with CNX and Blood
 Kidney Antibodies  >1 publications with CNX and Kidney
 Skin Antibodies  >1 publications with CNX and Skin
 Uterus Antibodies  >1 publications with CNX and Uterus
 Embryonic Tissue Antibodies  >1 publications with CNX and Embryonic Tissue
 Epididymis Antibodies  >1 publications with CNX and Epididymis
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions