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CPM elisa kit :: Human Carboxypeptidase M (CPM) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024622 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Carboxypeptidase M (CPM), ELISA Kit

 Also Known As   

Carboxypeptidase M (CPM) ELISA Kit

 Product Gene Name   

CPM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    114860
 3D Structure    ModBase 3D Structure for P14384
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase M (CPM).
No significant cross-reactivity or interference between Carboxypeptidase M (CPM) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    78.12-5000pg/mL
 Sensitivity    < 28pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Carboxypeptidase M (CPM) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Carboxypeptidase M (CPM) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CPM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CPM purchase    MBS2024622 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carboxypeptidase M (CPM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.12-5000pg/mL in biological research samples containing CPM, with an estimated sensitivity of < 28pg/mL. The ELISA analytical biochemical technique of the MBS2024622 kit is based on CPM antibody-CPM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CPM elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase M (CPM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase M (CPM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase M (CPM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase M (CPM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CPM elisa kit    Enzyme & Kinase; Tumor immunity; Infection immunity; Hormone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of CPM elisa kit    CPM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024622. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CPM. It may not necessarily be applicable to this product.
 NCBI GI #    53832021
 NCBI GeneID    1368
 NCBI Accession #    NP_001005502.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001005502.2 [Other Products]
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 UniProt Primary Accession #    P14384 [Other Products]
 UniProt Secondary Accession #    Q9H2K9; B2R800 [Other Products]
 UniProt Related Accession #    P14384 [Other Products]
 Molecular Weight    50,514 Da
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 NCBI Official Full Name    carboxypeptidase M
 NCBI Official Synonym Full Names    carboxypeptidase M
 NCBI Official Symbol    CPM [Similar Products]
 NCBI Protein Information    carboxypeptidase M
 UniProt Protein Name    Carboxypeptidase M
 Protein Family    Carboxypeptidase
 UniProt Gene Name    CPM [Similar Products]
 UniProt Synonym Gene Names    CPM [Similar Products]
 UniProt Entry Name    CBPM_HUMAN
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 NCBI Summary for CPM    The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CPM    CPM: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. Belongs to the peptidase M14 family.

Protein type: Membrane protein, GPI anchor; EC 3.4.17.12; Protease

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cell surface; plasma membrane

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: anatomical structure morphogenesis; proteolysis
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 Research Articles on CPM    1. CPM binding to extracellular loop 2 of the B1R results in positive allosteric modulation of B1R signaling, and disruption of this interaction could provide a novel therapeutic approach to reduce pathological B1R signaling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CPM elisa kitDiseases associated with CPM elisa kit
 Reference Product  PubMed Publications
 EGF elisa kit  >22 publications with CPM and EGF
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >468 publications with CPM and Nervous System Diseases
 Brain Diseases Antibodies  >269 publications with CPM and Brain Diseases
 Pain Antibodies  >268 publications with CPM and Pain
 Cardiovascular Diseases Antibodies  >231 publications with CPM and Cardiovascular Diseases
 Lung Diseases Antibodies  >136 publications with CPM and Lung Diseases
 Liver Diseases Antibodies  >134 publications with CPM and Liver Diseases
 Kidney Diseases Antibodies  >130 publications with CPM and Kidney Diseases
 Breast Neoplasms Antibodies  >127 publications with CPM and Breast Neoplasms
 Heart Diseases Antibodies  >99 publications with CPM and Heart Diseases
 Inflammation Antibodies  >94 publications with CPM and Inflammation
Organs/Tissues associated with CPM elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1246 publications with CPM and Blood
 Muscle Antibodies  >424 publications with CPM and Muscle
 Brain Antibodies  >374 publications with CPM and Brain
 Liver Antibodies  >329 publications with CPM and Liver
 Heart Antibodies  >275 publications with CPM and Heart
 Kidney Antibodies  >266 publications with CPM and Kidney
 Bone Antibodies  >251 publications with CPM and Bone
 Stomach Antibodies  >246 publications with CPM and Stomach
 Lung Antibodies  >215 publications with CPM and Lung
 Intestine Antibodies  >171 publications with CPM and Intestine
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