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cTnI elisa kit :: Human Cardiac Troponin I ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729403
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cardiac Troponin I (cTnI), ELISA Kit

 Also Known As   

Human Cardiac Troponin I ELISA Kit

 Product Gene Name   

cTnI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q6FGX2
 Species Reactivity    Human
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 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of cTnI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for cTnI purchase    MBS729403 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiac Troponin I (cTnI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing cTnI. The ELISA analytical biochemical technique of the MBS729403 kit is based on cTnI antibody-cTnI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect cTnI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, cTnI. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for cTnI elisa kit

   Intended Uses: This cTnI ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of cTnI in the sample, this cTnI ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus cTnI concentration. The concentration of cTnI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This cTnI enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for cTnI. Standards or samples are then added to the microtiter plate wells and cTnI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cTnI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cTnI are added to each well to "sandwich" the cTnI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain cTnI and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for cTnI elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of cTnI elisa kit    cTnI elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729403
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NCBI/Uniprot data below describe general gene information for cTnI. It may not necessarily be applicable to this product.
 NCBI GI #    49456923
 NCBI GeneID    7137
 NCBI Accession #    CAG46782.1 [Other Products]
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 UniProt Primary Accession #    Q6FGX2 [Other Products]
 UniProt Related Accession #    P19429 [Other Products]
 Molecular Weight    24,008 Da
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 NCBI Official Full Name    TNNI3
 NCBI Official Synonym Full Names    troponin I type 3 (cardiac)
 NCBI Official Symbol    TNNI3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
 UniProt Protein Name    TNNI3 protein
 UniProt Synonym Protein Names   
TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant
 UniProt Gene Name    TNNI3 [Similar Products]
 UniProt Entry Name    Q6FGX2_HUMAN
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 NCBI Summary for cTnI    Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
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 UniProt Comments for cTnI    TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
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 Research Articles on cTnI    1. Cardiac TnI release is common before Norwood and BDG operations; then during the treatment protocol for hypoplastic left heart syndrome, cTnI release resolves.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Human Cardiac Troponin I ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS340669  Anti-Cardiac Troponin I Antibody (Epitope 170-189) (Clone 6)  MBS596030  Recombinant human cardiac troponin I
 MBS190081  Cardiac Troponin I Monoclonal Antibody  MBS760944  Guinea pig cardiac Troponin I ELISA Kit
 MBS340626  Cardiac Troponin I (cTnI) epitope 55-70 clone 001.7B11  MBS2532163  Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit
 MBS285870  Rat Cardiac troponin I (cTn-I) ELISA Kit  MBS007065  Rabbit Cardiac Troponin I ELISA Kit
 MBS340671  Anti-Cardiac Troponin I Antibody (Epitope 21-39) (Clone 8)  MBS340627  Cardiac Troponin I (cTnI) epitope 80-100 clone 001.11D9
Pathways associated with cTnI elisa kitDiseases associated with cTnI elisa kit
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >71 publications with cTnI and Heart Diseases
 Cardiomyopathies Antibodies  >63 publications with cTnI and Cardiomyopathies
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >25 publications with cTnI and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >19 publications with cTnI and Cardiomyopathy, Dilated
 Cardiomyopathy, Restrictive Antibodies  >8 publications with cTnI and Cardiomyopathy, Restrictive
 Nervous System Diseases Antibodies  >5 publications with cTnI and Nervous System Diseases
 Hypertension Antibodies  >4 publications with cTnI and Hypertension
 Cardiomyopathy, Familial Restrictive, 1 Antibodies  >3 publications with cTnI and Cardiomyopathy, Familial Restrictive, 1
 Disease Models, Animal Antibodies  >2 publications with cTnI and Disease Models, Animal
Organs/Tissues associated with cTnI elisa kit
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >60 publications with cTnI and Heart
 Embryonic Tissue Antibodies  >2 publications with cTnI and Embryonic Tissue
 Lung Antibodies  >2 publications with cTnI and Lung
 Skin Antibodies  >1 publications with cTnI and Skin
 Uterus Antibodies  >1 publications with cTnI and Uterus
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