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CT-1 elisa kit :: Human Cardiotrophin-1 / CT-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355361
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $465 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cardiotrophin-1 / CT-1, ELISA Kit

 Also Known As   

CARDIOTROPHIN-1 / CT-1 Human ELISA Kit

 Product Synonym Names    Human Cardiotrophin-1 / CT-1
 Product Gene Name   

CT-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Chromosome Location    Chromosome: 16; NC_000016.9 (30907928..30914881). Location: 16p11.2
 OMIM    600435
 3D Structure    ModBase 3D Structure for Q16619
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 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    31.2 pg/ml-2000 pg/ml
 Sensitivity    < 10 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 2 months, or at -20 degree C for 4 months.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of CT-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CT-1 purchase    MBS355361 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiotrophin-1 / CT-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2 pg/ml-2000 pg/ml in biological research samples containing CT-1, with an estimated sensitivity of < 10 pg/ml. The ELISA analytical biochemical technique of the MBS355361 kit is based on CT-1 antibody-CT-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CT-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CT-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CT-1 elisa kit

   Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CT-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CT-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CT-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CT-1 can be calculated.

Background: Cardiotrophin-1 (CT-1) is a cytokine. CTF1 gene contains 3 exons, spans over 6 to 7 kb. It is a cardiac hypertrophic factor of 21.5 kDa and a protein member of the IL-6 cytokine family. It is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. This protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer.
 Applications Tested/Suitable for CT-1 elisa kit   

Quantitative sELISA (EIA)

 Application Notes for CT-1 elisa kit    For quantitative detection of CT-1 in human serum, body fluids, tissue lysates or cell culture supernatants.
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 Typical Testing Data/Standard Curve (for reference only) of CT-1 elisa kit    CT-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355361
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NCBI/Uniprot data below describe general gene information for CT-1. It may not necessarily be applicable to this product.
 NCBI GI #    216548056
 NCBI GeneID    1489
 NCBI Accession #    NP_001136016.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001142544.1 [Other Products]
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 UniProt Primary Accession #    Q16619 [Other Products]
 UniProt Secondary Accession #    Q5U5Y7; A8MVX4 [Other Products]
 UniProt Related Accession #    Q16619 [Other Products]
 Molecular Weight    21,227 Da
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 NCBI Official Full Name    cardiotrophin-1 isoform 2
 NCBI Official Synonym Full Names    cardiotrophin 1
 NCBI Official Symbol    CTF1 [Similar Products]
 NCBI Official Synonym Symbols   
CT1; CT-1
[Similar Products]
 NCBI Protein Information    cardiotrophin-1; cardiophin 1
 UniProt Protein Name    Cardiotrophin-1
 UniProt Gene Name    CTF1 [Similar Products]
 UniProt Synonym Gene Names    CT-1 [Similar Products]
 UniProt Entry Name    CTF1_HUMAN
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 NCBI Summary for CT-1    The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
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 UniProt Comments for CT-1    Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Subcellular location: Secreted.

Tissue specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.

Sequence similarities: Belongs to the IL-6 superfamily.
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 Research Articles on CT-1    1. Cardiotrophin-1 may serve as both a biomarker of left ventricular hypertrophy and dysfunction in hypertensive patients, and is potential target for therapies aimed to prevent and treat hypertensive heart disease beyond blood pressure control.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CT-1 elisa kitDiseases associated with CT-1 elisa kit
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with CT-1 and Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with CT-1 and Neoplasms, Experimental
 Neovascularization, Pathologic Antibodies  >1 publications with CT-1 and Neovascularization, Pathologic
 Hypertrophy Antibodies  >1 publications with CT-1 and Hypertrophy
 Reperfusion Injury Antibodies  >1 publications with CT-1 and Reperfusion Injury
 Acute Kidney Injury Antibodies  >1 publications with CT-1 and Acute Kidney Injury
 Heart Diseases Antibodies  >1 publications with CT-1 and Heart Diseases
 Inflammation Antibodies  >1 publications with CT-1 and Inflammation
 Ischemia Antibodies  >1 publications with CT-1 and Ischemia
 Necrosis Antibodies  >1 publications with CT-1 and Necrosis
Organs/Tissues associated with CT-1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with CT-1 and Brain
 Nerve Antibodies  >1 publications with CT-1 and Nerve
 Lung Antibodies  >1 publications with CT-1 and Lung
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