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CASP14 elisa kit :: Human Caspase 14 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720211
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Caspase 14 (CASP14), ELISA Kit

 Also Known As   

Human Caspase 14 ELISA Kit

 Product Gene Name   

CASP14 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS720211 MSDS    MSDS PDF
 OMIM    605848
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CASP14. No significant cross-reactivity or interference between CASP14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CASP14 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CASP14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CASP14 purchase    MBS720211 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase 14 (CASP14) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing CASP14, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS720211 kit is based on CASP14 antibody-CASP14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CASP14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CASP14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CASP14 elisa kit

   Intended Uses: This CASP14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CASP14. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CASP14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASP14 antibody and an CASP14-HRP conjugate. The assay sample and buffer are incubated together with CASP14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP14 concentration since CASP14 from samples and CASP14-HRP conjugate compete for the anti-CASP14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP14 from the sample, fewer sites are left to bind CASP14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP14 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CASP14 elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve of CASP14 elisa kit    CASP14 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS720211. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CASP14. It may not necessarily be applicable to this product.
 NCBI GI #    6912286
 NCBI GeneID    23581
 NCBI Accession #    NP_036246.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012114.2 [Other Products]
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 UniProt Secondary Accession #    O95823; Q3SYC9 [Other Products]
 UniProt Related Accession #    P31944 [Other Products]
 Molecular Weight    27,680 Da
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 NCBI Official Full Name    caspase-14
 NCBI Official Synonym Full Names    caspase 14, apoptosis-related cysteine peptidase
 NCBI Official Symbol    CASP14 [Similar Products]
 NCBI Protein Information    caspase-14; CASP-14; apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine protease
 UniProt Protein Name    Caspase-14
 Protein Family    Caspase
 UniProt Gene Name    CASP14 [Similar Products]
 UniProt Synonym Gene Names    CASP-14 [Similar Products]
 UniProt Entry Name    CASPE_HUMAN
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 NCBI Summary for CASP14    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CASP14    CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.-; Protease

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cytoplasm; keratin filament; nucleus

Molecular Function: cysteine-type endopeptidase activity

Biological Process: epidermis development; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; proteolysis
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 Research Articles on CASP14    1. partial loss of caspase 14 is not associated with dedifferentiation in neoplastic lesions of the oral mucosa
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CASP14 elisa kitDiseases associated with CASP14 elisa kit
 Reference Product  PubMed Publications
 CASP8 elisa kit  >4 publications with CASP14 and CASP8
 CASP10 elisa kit  >2 publications with CASP14 and CASP10
 CASP1 elisa kit  >2 publications with CASP14 and CASP1
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >5 publications with CASP14 and Brain Diseases
 Skin Neoplasms Antibodies  >4 publications with CASP14 and Skin Neoplasms
 Cardiovascular Diseases Antibodies  >3 publications with CASP14 and Cardiovascular Diseases
 Inflammation Antibodies  >3 publications with CASP14 and Inflammation
 Necrosis Antibodies  >2 publications with CASP14 and Necrosis
 Nerve Degeneration Antibodies  >1 publications with CASP14 and Nerve Degeneration
 Liver Neoplasms Antibodies  >1 publications with CASP14 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with CASP14 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >1 publications with CASP14 and Lung Neoplasms
 Colonic Neoplasms Antibodies  >1 publications with CASP14 and Colonic Neoplasms
Organs/Tissues associated with CASP14 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >33 publications with CASP14 and Skin
 Thymus Antibodies  >1 publications with CASP14 and Thymus
 Lymph Node Antibodies  >1 publications with CASP14 and Lymph Node
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