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CDC42EP4 elisa kit :: Human Cdc42 effector protein 4 (CDC42EP4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7239514
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
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 Product Name   

Cdc42 effector protein 4 (CDC42EP4), ELISA Kit

★Popular Item★
 Also Known As   

Human Cdc42 effector protein 4 (CDC42EP4) ELISA Kit

 Product Gene Name   

CDC42EP4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605468
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CDC42EP4. No significant cross-reactivity or interference between CDC42EP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC42EP4 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CDC42EP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CDC42EP4 purchase    MBS7239514 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cdc42 effector protein 4 (CDC42EP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing CDC42EP4, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7239514 kit is based on CDC42EP4 antibody-CDC42EP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDC42EP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDC42EP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CDC42EP4 elisa kit

   Intended Uses: This CDC42EP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDC42EP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||CDC42EP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC42EP4 antibody and an CDC42EP4-HRP conjugate. The assay sample and buffer are incubated together with CDC42EP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC42EP4 concentration since CDC42EP4 from samples and CDC42EP4-HRP conjugate compete for the anti-CDC42EP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC42EP4 from the sample, fewer sites are left to bind CDC42EP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC42EP4 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CDC42EP4 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve of CDC42EP4 elisa kit    CDC42EP4 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS7239514. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CDC42EP4. It may not necessarily be applicable to this product.
 NCBI GI #    13786127
 NCBI GeneID    23580
 NCBI Accession #    NP_036253.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012121.4 [Other Products]
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 UniProt Secondary Accession #    O95828; Q96FT3; B3KUS7 [Other Products]
 UniProt Related Accession #    Q9H3Q1 [Other Products]
 Molecular Weight    30,214 Da
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 NCBI Official Full Name    cdc42 effector protein 4
 NCBI Official Synonym Full Names    CDC42 effector protein (Rho GTPase binding) 4
 NCBI Official Symbol    CDC42EP4 [Similar Products]
 NCBI Official Synonym Symbols   
CEP4; BORG4; KAIA1777
[Similar Products]
 NCBI Protein Information    cdc42 effector protein 4; binder of Rho GTPases 4
 UniProt Protein Name    Cdc42 effector protein 4
 UniProt Synonym Protein Names   
Binder of Rho GTPases 4
 Protein Family    Cdc42 effector protein
 UniProt Gene Name    CDC42EP4 [Similar Products]
 UniProt Synonym Gene Names    BORG4; CEP4 [Similar Products]
 UniProt Entry Name    BORG4_HUMAN
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 NCBI Summary for CDC42EP4    The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CDC42EP4    CDC42EP4: Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation, when overexpressed in fibroblasts. Belongs to the BORG/CEP family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q24-q25

Cellular Component: microtubule cytoskeleton; cytoplasm; plasma membrane; endomembrane system; actin cytoskeleton

Molecular Function: GTP-Rho binding

Biological Process: regulation of cell shape; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; Rho protein signal transduction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CDC42EP4 elisa kitDiseases associated with CDC42EP4 elisa kit
 Reference Product  PubMed Publications
 CDC42 elisa kit  >12 publications with CDC42EP4 and CDC42
 RHOQ elisa kit  >1 publications with CDC42EP4 and RHOQ
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with CDC42EP4 and Nervous System Diseases
Organs/Tissues associated with CDC42EP4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with CDC42EP4 and Blood
 Brain Antibodies  >3 publications with CDC42EP4 and Brain
 Bone Antibodies  >1 publications with CDC42EP4 and Bone
 Nerve Antibodies  >1 publications with CDC42EP4 and Nerve
 Liver Antibodies  >1 publications with CDC42EP4 and Liver
 Spleen Antibodies  >1 publications with CDC42EP4 and Spleen
 Vascular Antibodies  >1 publications with CDC42EP4 and Vascular
 Embryonic Tissue Antibodies  >1 publications with CDC42EP4 and Embryonic Tissue
 Kidney Antibodies  >1 publications with CDC42EP4 and Kidney
 Lung Antibodies  >1 publications with CDC42EP4 and Lung
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