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GPA33 elisa kit :: Human Cell surface A33 antigen ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761547
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cell surface A33 antigen (GPA33), ELISA Kit

★Popular Item★
 Also Known As   

Human Cell surface A33 antigen ELISA Kit

 Product Synonym Names    GPA33/A33/Glycoprotein A33/glycoprotein A33 (transmembrane)/transmembrane glycoprotein A33
 Product Gene Name   

GPA33 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS761547 COA    COA PDF
 OMIM    602171
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GPA33. No significant cross-reactivity or interference between GPA33 and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.625-40ng/ml
 Sensitivity    <0.375ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GPA33 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GPA33 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPA33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPA33 purchase    MBS761547 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cell surface A33 antigen (GPA33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/ml in biological research samples containing GPA33, with an estimated sensitivity of <0.375ng/ml. The ELISA analytical biochemical technique of the MBS761547 kit is based on GPA33 antibody-GPA33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPA33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPA33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPA33 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GPA33 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GPA33 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GPA33 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GPA33 can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of GPA33 elisa kit    GPA33 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS761547. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GPA33. It may not necessarily be applicable to this product.
 NCBI GI #    5031561
 NCBI GeneID    10223
 NCBI Accession #    NP_005805.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005814.2 [Other Products]
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 UniProt Secondary Accession #    Q5VZP6 [Other Products]
 UniProt Related Accession #    Q99795 [Other Products]
 Molecular Weight    35,632 Da
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 NCBI Official Full Name    cell surface A33 antigen
 NCBI Official Synonym Full Names    glycoprotein A33
 NCBI Official Symbol    GPA33 [Similar Products]
 NCBI Official Synonym Symbols   
A33
[Similar Products]
 NCBI Protein Information    cell surface A33 antigen
 UniProt Protein Name    Cell surface A33 antigen
 UniProt Synonym Protein Names   
Glycoprotein A33
 Protein Family    Cell surface A33 antigen
 UniProt Gene Name    GPA33 [Similar Products]
 UniProt Entry Name    GPA33_HUMAN
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 NCBI Summary for GPA33    The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GPA33    GPA33: May play a role in cell-cell recognition and signaling.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q24.1

Cellular Component: cell surface; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; receptor activity

Biological Process: cell-cell adhesion
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 Research Articles on GPA33    1. Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with GPA33 elisa kitOrgans/Tissues associated with GPA33 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >8 publications with GPA33 and Neoplasms, Experimental
 Neutropenia Antibodies  >3 publications with GPA33 and Neutropenia
 Necrosis Antibodies  >3 publications with GPA33 and Necrosis
 Inflammation Antibodies  >2 publications with GPA33 and Inflammation
 Disease Models, Animal Antibodies  >2 publications with GPA33 and Disease Models, Animal
 Liver Neoplasms Antibodies  >2 publications with GPA33 and Liver Neoplasms
 Hepatitis Antibodies  >1 publications with GPA33 and Hepatitis
 Liver Neoplasms, Experimental Antibodies  >1 publications with GPA33 and Liver Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >17 publications with GPA33 and Intestine
 Liver Antibodies  >5 publications with GPA33 and Liver
 Stomach Antibodies  >4 publications with GPA33 and Stomach
 Kidney Antibodies  >4 publications with GPA33 and Kidney
 Lung Antibodies  >2 publications with GPA33 and Lung
 Testis Antibodies  >2 publications with GPA33 and Testis
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