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CCR5 elisa kit :: Human Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020083 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $515 +1 FREE 8GB USB
96-Strip-Wells  /  $695 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,685 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,850 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Chemokine C-C-Motif Receptor 5 (CCR5), ELISA Kit

 Also Known As   

Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit

 Product Synonym Names    CD195; CC-CKR5; CCCKR5; CMKBR5; CHEMR13; HIV-1 fusion coreceptor
 Product Gene Name   

CCR5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    601373
 3D Structure    ModBase 3D Structure for P51681
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 5 (CCR5).
No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 5 (CCR5) and analogues was observed.
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 Samples    Tissue homogenates, Cell lysates and other biological fluids
 Assay Type    Sandwich
 Detection Range    15.63-1000pg/mL
 Sensitivity    Typically less than 6.0pg/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 5 (CCR5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 5 (CCR5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Other Notes    Small volumes of CCR5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CCR5 purchase    MBS2020083 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chemokine C-C-Motif Receptor 5 (CCR5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.63-1000pg/mL in biological research samples containing CCR5, with an estimated sensitivity of Typically less than 6.0pg/mL. The ELISA analytical biochemical technique of the MBS2020083 kit is based on CCR5 antibody-CCR5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCR5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCR5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CCR5 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 5 (CCR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 5 (CCR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 5 (CCR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 5 (CCR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CCR5 elisa kit    CCR5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020083
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NCBI/Uniprot data below describe general gene information for CCR5. It may not necessarily be applicable to this product.
 NCBI GI #    28461320
 NCBI GeneID    1234
 NCBI Accession #    AAH38398.1 [Other Products]
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 UniProt Primary Accession #    P51681 [Other Products]
 UniProt Secondary Accession #    O14692; O14693; O14695; O14696; O14697; O14698; O14699; O14700; O14701; O14702; O14703 [Other Products]
 UniProt Related Accession #    P51681 [Other Products]
 Molecular Weight    40,524 Da
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 NCBI Official Full Name    Chemokine (C-C motif) receptor 5
 NCBI Official Synonym Full Names    chemokine (C-C motif) receptor 5 (gene/pseudogene)
 NCBI Official Symbol    CCR5 [Similar Products]
 NCBI Official Synonym Symbols   
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
[Similar Products]
 NCBI Protein Information    C-C chemokine receptor type 5
 UniProt Protein Name    C-C chemokine receptor type 5
 UniProt Synonym Protein Names   
CHEMR13; HIV-1 fusion coreceptor; CD_antigen: CD195
 Protein Family    C-C chemokine receptor
 UniProt Gene Name    CCR5 [Similar Products]
 UniProt Synonym Gene Names    CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5 [Similar Products]
 UniProt Entry Name    CCR5_HUMAN
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 NCBI Summary for CCR5    This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2015]
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 UniProt Comments for CCR5    CCR5: a 7-transmembrane G-linked receptor for a number of inflammatory C-C type chemokines including MIP-1-alpha, MIP-1-beta and RANTES. Transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (along with CD4) for HIV-1 R5 isolates. Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCR4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on S6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1). Variations in CCR5 gene also influence the rate of progression to AIDS after infection. R60S variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors. Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein binding; C-C chemokine receptor activity; chemokine receptor activity; C-C chemokine binding; coreceptor activity; actin binding; phosphoinositide phospholipase C activity

Biological Process: viral reproduction; calcium-mediated signaling; MAPKKK cascade; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; calcium ion transport; cellular defense response; dendritic cell chemotaxis; entry into host cell; immune response; inflammatory response

Disease: West Nile Virus, Susceptibility To; Diabetes Mellitus, Insulin-dependent, 22; Hepatitis C Virus, Susceptibility To
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 Research Articles on CCR5    1. Polymorphisms in the HIV gp120 bridging sheet enhanced gp120-CCR5 interactions, thereby promoting infection of brain.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CCR5 elisa kitDiseases associated with CCR5 elisa kit
 Products by Pathway  Pathway Diagram
 Binding And Entry Of HIV Virion Pathway antibodies  Binding And Entry Of HIV Virion Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >873 publications with CCR5 and Inflammation
 Nervous System Diseases Antibodies  >406 publications with CCR5 and Nervous System Diseases
 Necrosis Antibodies  >283 publications with CCR5 and Necrosis
 Cardiovascular Diseases Antibodies  >232 publications with CCR5 and Cardiovascular Diseases
 Liver Diseases Antibodies  >200 publications with CCR5 and Liver Diseases
 Lung Diseases Antibodies  >189 publications with CCR5 and Lung Diseases
 Hypersensitivity Antibodies  >136 publications with CCR5 and Hypersensitivity
 Hepatitis C Antibodies  >116 publications with CCR5 and Hepatitis C
 Fibrosis Antibodies  >97 publications with CCR5 and Fibrosis
 Kidney Diseases Antibodies  >96 publications with CCR5 and Kidney Diseases
Organs/Tissues associated with CCR5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3812 publications with CCR5 and Blood
 Brain Antibodies  >364 publications with CCR5 and Brain
 Lymph Node Antibodies  >261 publications with CCR5 and Lymph Node
 Kidney Antibodies  >180 publications with CCR5 and Kidney
 Thymus Antibodies  >109 publications with CCR5 and Thymus
 Muscle Antibodies  >81 publications with CCR5 and Muscle
 Eye Antibodies  >40 publications with CCR5 and Eye
 Pharynx Antibodies  >30 publications with CCR5 and Pharynx
 Connective Tissue Antibodies  >27 publications with CCR5 and Connective Tissue
 Prostate Antibodies  >27 publications with CCR5 and Prostate
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