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CHIT1 elisa kit :: Human Chitotriosidase-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533435
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
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 Product Name   

Chitotriosidase-1 (CHIT1), ELISA Kit

★Popular Item★
 Also Known As   

Human CHIT1 (Chitotriosidase-1) ELISA Kit

 Product Gene Name   

CHIT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2533435 COA    COA PDF
 OMIM    600031
 3D Structure    ModBase 3D Structure for Q13231
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanCHIT1. No significant cross-reactivity or interference between HumanCHIT1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    78.125-5000pg/mL
 Sensitivity    Min: 46.875pg/mL; Max: 5000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CHIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHIT1 purchase    MBS2533435 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chitotriosidase-1 (CHIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.125-5000pg/mL in biological research samples containing CHIT1, with an estimated sensitivity of Min: 46.875pg/mL; Max: 5000pg/mL. The ELISA analytical biochemical technique of the MBS2533435 kit is based on CHIT1 antibody-CHIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CHIT1 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanCHIT1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CHIT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CHIT1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CHIT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CHIT1.You can calculate the concentration of CHIT1in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CHIT1 elisa kit    CHIT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2533435. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.
 NCBI GI #    4502809
 NCBI GeneID    1118
 NCBI Accession #    NP_003456.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003465.2 [Other Products]
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 UniProt Primary Accession #    Q13231 [Other Products]
 UniProt Secondary Accession #    Q0VGG5; Q0VGG6; Q3ZAR1; Q6ISC2; Q9H3V8; Q9UDJ8; B3KNW6; J3KN09 [Other Products]
 UniProt Related Accession #    Q13231 [Other Products]
 Molecular Weight    49,613 Da
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 NCBI Official Full Name    chitotriosidase-1 isoform 1
 NCBI Official Synonym Full Names    chitinase 1
 NCBI Official Symbol    CHIT1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    chitotriosidase-1
 UniProt Protein Name    Chitotriosidase-1
 UniProt Synonym Protein Names   
 Protein Family    Chitotriosidase
 UniProt Gene Name    CHIT1 [Similar Products]
 UniProt Entry Name    CHIT1_HUMAN
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 NCBI Summary for CHIT1    Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for CHIT1    CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC; Hydrolase; Secreted; Secreted, signal peptide; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; extracellular space; lysosome

Molecular Function: chitin binding; chitinase activity; endochitinase activity

Biological Process: carbohydrate metabolic process; chitin catabolic process; immune response; polysaccharide catabolic process; response to bacterium

Disease: Chitotriosidase Deficiency
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 Research Articles on CHIT1    1. BALF chitotriosidase activity is increased in patients with sarcoidosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHIT1 elisa kitDiseases associated with CHIT1 elisa kit
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >15 publications with CHIT1 and Lung Diseases
 Gaucher Disease Antibodies  >14 publications with CHIT1 and Gaucher Disease
 Inflammation Antibodies  >13 publications with CHIT1 and Inflammation
 Nervous System Diseases Antibodies  >8 publications with CHIT1 and Nervous System Diseases
 Brain Diseases Antibodies  >7 publications with CHIT1 and Brain Diseases
 Fibrosis Antibodies  >6 publications with CHIT1 and Fibrosis
 Necrosis Antibodies  >3 publications with CHIT1 and Necrosis
 Kidney Diseases Antibodies  >2 publications with CHIT1 and Kidney Diseases
 Disease Models, Animal Antibodies  >1 publications with CHIT1 and Disease Models, Animal
 Bone Marrow Diseases Antibodies  >1 publications with CHIT1 and Bone Marrow Diseases
Organs/Tissues associated with CHIT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with CHIT1 and Blood
 Lung Antibodies  >12 publications with CHIT1 and Lung
 Heart Antibodies  >3 publications with CHIT1 and Heart
 Bone Marrow Antibodies  >1 publications with CHIT1 and Bone Marrow
 Brain Antibodies  >1 publications with CHIT1 and Brain
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