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CHIT1 elisa kit :: Human Chitotriosidase-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765849
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Chitotriosidase-1 (CHIT1), ELISA Kit

★Popular Item★
 Also Known As   

Human Chitotriosidase-1 ELISA Kit

 Product Synonym Names    CHIT1/Chitotriosidase-1/Chitinase-1/CHI3/CHIT/plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase/FLJ00314/MGC125322
 Product Gene Name   

CHIT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765849 COA    COA PDF
 OMIM    600031
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CHIT1. No significant cross-reactivity or interference between CHIT1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHIT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHIT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CHIT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CHIT1 purchase    MBS765849 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chitotriosidase-1 (CHIT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing CHIT1, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS765849 kit is based on CHIT1 antibody-CHIT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHIT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHIT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CHIT1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CHIT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CHIT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CHIT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CHIT1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of CHIT1 elisa kit    CHIT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS765849. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CHIT1. It may not necessarily be applicable to this product.
 NCBI GI #    4502809
 NCBI GeneID    1118
 NCBI Accession #    NP_003456.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003465.2 [Other Products]
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 UniProt Secondary Accession #    Q0VGG5; Q0VGG6; Q3ZAR1; Q6ISC2; Q9H3V8; Q9UDJ8; B3KNW6; J3KN09 [Other Products]
 UniProt Related Accession #    Q13231 [Other Products]
 Molecular Weight    49,613 Da
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 NCBI Official Full Name    chitotriosidase-1 isoform 1
 NCBI Official Synonym Full Names    chitinase 1
 NCBI Official Symbol    CHIT1 [Similar Products]
 NCBI Official Synonym Symbols   
CHI3; CHIT; CHITD
[Similar Products]
 NCBI Protein Information    chitotriosidase-1
 UniProt Protein Name    Chitotriosidase-1
 UniProt Synonym Protein Names   
Chitinase-1
 Protein Family    Chitotriosidase
 UniProt Gene Name    CHIT1 [Similar Products]
 UniProt Entry Name    CHIT1_HUMAN
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 NCBI Summary for CHIT1    Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for CHIT1    CHIT1: Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Hydrolase; Secreted; EC 3.2.1.14; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; extracellular space; lysosome

Molecular Function: chitin binding; chitinase activity; endochitinase activity

Biological Process: carbohydrate metabolic process; chitin catabolic process; immune response; polysaccharide catabolic process; response to bacterium

Disease: Chitotriosidase Deficiency
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 Research Articles on CHIT1    1. The two Chit1 isoforms combine features of exo-processive and endo-nonprocessive chitinases with the somewhat unusual carbohydrate binding module 14 to reach a high degree of efficiency.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CHIT1 elisa kitDiseases associated with CHIT1 elisa kit
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >15 publications with CHIT1 and Lung Diseases
 Gaucher Disease Antibodies  >14 publications with CHIT1 and Gaucher Disease
 Inflammation Antibodies  >13 publications with CHIT1 and Inflammation
 Nervous System Diseases Antibodies  >8 publications with CHIT1 and Nervous System Diseases
 Brain Diseases Antibodies  >7 publications with CHIT1 and Brain Diseases
 Fibrosis Antibodies  >6 publications with CHIT1 and Fibrosis
 Necrosis Antibodies  >3 publications with CHIT1 and Necrosis
 Kidney Diseases Antibodies  >2 publications with CHIT1 and Kidney Diseases
 Disease Models, Animal Antibodies  >1 publications with CHIT1 and Disease Models, Animal
 Bone Marrow Diseases Antibodies  >1 publications with CHIT1 and Bone Marrow Diseases
Organs/Tissues associated with CHIT1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >25 publications with CHIT1 and Blood
 Lung Antibodies  >12 publications with CHIT1 and Lung
 Heart Antibodies  >3 publications with CHIT1 and Heart
 Bone Marrow Antibodies  >1 publications with CHIT1 and Bone Marrow
 Brain Antibodies  >1 publications with CHIT1 and Brain
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