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CMA1 elisa kit :: Human Chymase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761959
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Chymase (CMA1), ELISA Kit

★Popular Item★
 Also Known As   

Human Chymase ELISA Kit

 Product Synonym Names    CMA1 (Chymase)/Alpha-chymase/Mast cell protease I
 Product Gene Name   

CMA1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS761959 COA    COA PDF
 OMIM    118938
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CMA1. No significant cross-reactivity or interference between CMA1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.312-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CMA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CMA1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CMA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CMA1 purchase    MBS761959 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chymase (CMA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/ml in biological research samples containing CMA1, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS761959 kit is based on CMA1 antibody-CMA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CMA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CMA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CMA1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CMA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CMA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CMA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CMA1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of CMA1 elisa kit    CMA1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS761959. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CMA1. It may not necessarily be applicable to this product.
 NCBI GI #    4502907
 NCBI GeneID    1215
 NCBI Accession #    NP_001827.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001836.4 [Other Products]
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 UniProt Secondary Accession #    Q16018; Q3SY36; Q3SY37; Q9UDH5; B5BUM8 [Other Products]
 UniProt Related Accession #    P23946 [Other Products]
 Molecular Weight    14,893 Da
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 NCBI Official Full Name    chymase isoform 1 preproprotein
 NCBI Official Synonym Full Names    chymase 1, mast cell
 NCBI Official Symbol    CMA1 [Similar Products]
 NCBI Official Synonym Symbols   
CYH; MCT1; chymase
[Similar Products]
 NCBI Protein Information    chymase
 UniProt Protein Name    Chymase
 UniProt Synonym Protein Names   
Alpha-chymase; Mast cell protease I
 Protein Family    Chymase
 UniProt Gene Name    CMA1 [Similar Products]
 UniProt Synonym Gene Names    CYH; CYM [Similar Products]
 UniProt Entry Name    CMA1_HUMAN
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 NCBI Summary for CMA1    This gene encodes a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants. [provided by RefSeq, Apr 2015]
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 UniProt Comments for CMA1    CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.39

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: cytoplasm; extracellular matrix; extracellular region; intracellular membrane-bound organelle

Molecular Function: peptide binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: angiotensin maturation; cellular protein metabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; immune response; interleukin-1 beta biosynthetic process; midbrain development; positive regulation of angiogenesis; protein processing; regulation of inflammatory response
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 Research Articles on CMA1    1. Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CMA1 elisa kitPathways associated with CMA1 elisa kit
 Reference Product  PubMed Publications
 AGT elisa kit  >6 publications with CMA1 and AGT
 CPA3 elisa kit  >4 publications with CMA1 and CPA3
 REN elisa kit  >2 publications with CMA1 and REN
 IL4 elisa kit  >1 publications with CMA1 and IL4
 ACE2 elisa kit  >1 publications with CMA1 and ACE2
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with CMA1 elisa kitOrgans/Tissues associated with CMA1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >12 publications with CMA1 and Inflammation
 Vascular Diseases Antibodies  >12 publications with CMA1 and Vascular Diseases
 Fibrosis Antibodies  >10 publications with CMA1 and Fibrosis
 Hypertension Antibodies  >9 publications with CMA1 and Hypertension
 Hypertrophy Antibodies  >5 publications with CMA1 and Hypertrophy
 Necrosis Antibodies  >4 publications with CMA1 and Necrosis
 Hypertrophy, Left Ventricular Antibodies  >2 publications with CMA1 and Hypertrophy, Left Ventricular
 Cell Transformation, Neoplastic Antibodies  >2 publications with CMA1 and Cell Transformation, Neoplastic
 Lung Injury Antibodies  >2 publications with CMA1 and Lung Injury
 Pulmonary Fibrosis Antibodies  >2 publications with CMA1 and Pulmonary Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >1 publications with CMA1 and Mixed
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