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CLU elisa kit :: Human Clusterin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176539
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Clusterin, ELISA Kit

★Popular Item★
 Also Known As   

Human Clusterin PicoKine ELISA Kit

 Product Synonym Names    Clusterin; clusterin; AAG4; Aging associated protein 4; APO J; APOJ; CLI; CLU; CLU1; CLU2; Clusterin; Complement associated protein SP 40,40; Complement cytolysis inhibitor; Complement lysis inhibitor; Ku70 binding protein 1; KUB1; NA1/NA2; SGP 2; SGP2; SP 40; SP40; Sulfated glycoprotein 2; Testosterone repressed prostate message 2; TRPM 2; TRPM2
 Product Gene Name   

CLU elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    185430
 3D Structure    ModBase 3D Structure for P10909
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 Species Reactivity    Human
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 Samples    cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CLU elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CLU purchase    MBS176539 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Clusterin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLU. The ELISA analytical biochemical technique of the MBS176539 kit is based on CLU antibody-CLU antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLU antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLU. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CLU elisa kit

   Principle of the Assay: The Picokine™ Human CLU Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CLU with a 96-well strip plate that is pre-coated with antibody specific for CLU. The detection antibody is a biotinylated antibody specific for CLU. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CLU with immunogen: Expression system for standard: NSO; Immunogen sequence: D23-R227(beta)&S228-E449(alpha). The kit is analytically validated with ready to use reagents. To measure Human CLU, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CLU in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CLU in the sample. For more information on assay principle, protocols, and troubleshooting tips.

Background: Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate noncaspase- dependent neuronal death following acute brain injury.
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 Typical Testing Data/Standard Curve (for reference only) of CLU elisa kit    CLU elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176539. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CLU. It may not necessarily be applicable to this product.
 NCBI GI #    116533
 NCBI GeneID    1191
 NCBI Accession #    P10909.1 [Other Products]
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 UniProt Primary Accession #    P10909 [Other Products]
 UniProt Secondary Accession #    P11380; P11381; Q2TU75; Q5HYC1; Q7Z5B9; B2R9Q1; B3KSE6 [Other Products]
 UniProt Related Accession #    P10909; AAB25217 [Other Products]
 Molecular Weight    53,643 Da
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 NCBI Official Full Name    Clusterin
 NCBI Official Synonym Full Names    clusterin
 NCBI Official Symbol    CLU [Similar Products]
 NCBI Official Synonym Symbols   
CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
[Similar Products]
 NCBI Protein Information    clusterin; apolipoprotein J; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2
 UniProt Protein Name    Clusterin
 UniProt Synonym Protein Names   
Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate message 2; TRPM-2
 Protein Family    Clusterin
 UniProt Gene Name    CLU [Similar Products]
 UniProt Synonym Gene Names    APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2 [Similar Products]
 UniProt Entry Name    CLUS_HUMAN
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 NCBI Summary for CLU    The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
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 UniProt Comments for CLU    CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Mitochondrial; Secreted, signal peptide; Apoptosis

Chromosomal Location of Human Ortholog: 8p21-p12

Cellular Component: extracellular matrix; Golgi apparatus; extracellular space; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; mitochondrial membrane; extracellular region; nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; misfolded protein binding

Biological Process: platelet activation; response to misfolded protein; release of cytochrome c from mitochondria; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; activation of NF-kappaB transcription factor; negative regulation of protein homooligomerization; complement activation; platelet degranulation; reverse cholesterol transport; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; innate immune response; lipid metabolic process; chaperone-mediated protein complex assembly; blood coagulation; complement activation, classical pathway
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 Research Articles on CLU    1. The CLU-1 isoform is the predominant growth-suppressive variant in head and neck squamous cell carcinoma and is targeted by miRNA-21.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CLU elisa kitPathways associated with CLU elisa kit
 Reference Product  PubMed Publications
 APP elisa kit  >20 publications with CLU and APP
 TGFB1 elisa kit  >15 publications with CLU and TGFB1
 EGF elisa kit  >14 publications with CLU and EGF
 UBC elisa kit  >6 publications with CLU and UBC
 FN1 elisa kit  >2 publications with CLU and FN1
 VHL elisa kit  >1 publications with CLU and VHL
 ALB elisa kit  >1 publications with CLU and ALB
 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram
Diseases associated with CLU elisa kitOrgans/Tissues associated with CLU elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >195 publications with CLU and Nervous System Diseases
 Brain Diseases Antibodies  >176 publications with CLU and Brain Diseases
 Alzheimer Disease Antibodies  >144 publications with CLU and Alzheimer Disease
 Prostatic Neoplasms Antibodies  >86 publications with CLU and Prostatic Neoplasms
 Kidney Diseases Antibodies  >61 publications with CLU and Kidney Diseases
 Cardiovascular Diseases Antibodies  >59 publications with CLU and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >54 publications with CLU and Disease Models, Animal
 Adenocarcinoma Antibodies  >50 publications with CLU and Adenocarcinoma
 Breast Neoplasms Antibodies  >48 publications with CLU and Breast Neoplasms
 Necrosis Antibodies  >48 publications with CLU and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >251 publications with CLU and Blood
 Brain Antibodies  >183 publications with CLU and Brain
 Prostate Antibodies  >128 publications with CLU and Prostate
 Kidney Antibodies  >87 publications with CLU and Kidney
 Nerve Antibodies  >80 publications with CLU and Nerve
 Vascular Antibodies  >70 publications with CLU and Vascular
 Liver Antibodies  >67 publications with CLU and Liver
 Eye Antibodies  >45 publications with CLU and Eye
 Testis Antibodies  >44 publications with CLU and Testis
 Muscle Antibodies  >36 publications with CLU and Muscle
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