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COL1a1 elisa kit :: Human Collagen Type I Alpha 1 (COL1a1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023330 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Collagen Type I Alpha 1 (COL1a1), ELISA Kit

★Popular Item★
 Also Known As   

Collagen Type I Alpha 1 (COL1a1) ELISA Kit

 Product Synonym Names    COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
 Product Gene Name   

COL1a1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q6LAN8
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1).
No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    31.2-2000pg/mL
 Sensitivity    < 12.8pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL1a1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL1a1 purchase    MBS2023330 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type I Alpha 1 (COL1a1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000pg/mL in biological research samples containing COL1a1, with an estimated sensitivity of < 12.8pg/mL. The ELISA analytical biochemical technique of the MBS2023330 kit is based on COL1a1 antibody-COL1a1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL1a1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL1a1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL1a1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 1 (COL1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 1 (COL1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 1 (COL1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 1 (COL1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for COL1a1 elisa kit    Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of COL1a1 elisa kit    COL1a1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023330. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL1a1. It may not necessarily be applicable to this product.
 NCBI GI #    1888409
 NCBI Accession #    CAA67261.1 [Other Products]
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 UniProt Primary Accession #    Q6LAN8 [Other Products]
 Molecular Weight    97,445 Da
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 NCBI Official Full Name    collagen type I alpha 1, partial
 UniProt Protein Name    Collagen type I alpha 1
 UniProt Synonym Protein Names   
Collagen type I alpha 1
 Protein Family    Collagen
 UniProt Gene Name    COL1A1 [Similar Products]
 UniProt Entry Name    Q6LAN8_HUMAN
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with COL1a1 elisa kit
 Reference Product  PubMed Publications
 COL1A2 elisa kit  >346 publications with COL1a1 and COL1A2
 SPARC elisa kit  >35 publications with COL1a1 and SPARC
 FN1 elisa kit  >34 publications with COL1a1 and FN1
 SP7 elisa kit  >27 publications with COL1a1 and SP7
 LEPRE1 elisa kit  >21 publications with COL1a1 and LEPRE1
 COL4A5 elisa kit  >7 publications with COL1a1 and COL4A5
 COL7A1 elisa kit  >7 publications with COL1a1 and COL7A1
 ITGB1 elisa kit  >6 publications with COL1a1 and ITGB1
 P4HB elisa kit  >1 publications with COL1a1 and P4HB
 ITGA2 elisa kit  >1 publications with COL1a1 and ITGA2
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