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COL1 elisa kit :: Human Collagen Type I (COL1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019319 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Collagen Type I (COL1), ELISA Kit

★Popular Item★
 Also Known As   

Collagen Type I (COL1) ELISA Kit

 Product Gene Name   

COL1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    120160
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Collagen Type I (COL1).
No significant cross-reactivity or interference between Collagen Type I (COL1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    62.5-4,000pg/mL
 Sensitivity    < 26.8pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Collagen Type I (COL1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Collagen Type I (COL1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL1 purchase    MBS2019319 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type I (COL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4,000pg/mL in biological research samples containing COL1, with an estimated sensitivity of < 26.8pg/mL. The ELISA analytical biochemical technique of the MBS2019319 kit is based on COL1 antibody-COL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I (COL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I (COL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I (COL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I (COL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for COL1 elisa kit    Metabolic pathway; Developmental science; Bone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of COL1 elisa kit    COL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019319. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL1. It may not necessarily be applicable to this product.
 NCBI GI #    45708783
 NCBI GeneID    1278
 NCBI Accession #    AAH42586.1 [Other Products]
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 UniProt Secondary Accession #    P02464; Q13897; Q13997; Q13998; Q14038; Q14057; Q15177; Q15947; Q16480; Q16511; Q7Z5S6 [Other Products]
 UniProt Related Accession #    P08123 [Other Products]
 Molecular Weight    129,314 Da
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 NCBI Official Full Name    Collagen, type I, alpha 2
 NCBI Official Synonym Full Names    collagen, type I, alpha 2
 NCBI Official Symbol    COL1A2 [Similar Products]
 NCBI Official Synonym Symbols   
OI4
[Similar Products]
 NCBI Protein Information    collagen alpha-2(I) chain
 UniProt Protein Name    Collagen alpha-2(I) chain
 UniProt Synonym Protein Names   
Alpha-2 type I collagen
 Protein Family    Zinc finger protein CONSTANS
 UniProt Gene Name    COL1A2 [Similar Products]
 UniProt Entry Name    CO1A2_HUMAN
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 NCBI Summary for COL1    This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
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 UniProt Comments for COL1    COL1A2: Type I collagen is a member of group I collagen (fibrillar forming collagen). Defects in COL1A2 are the cause of Ehlers-Danlos syndrome type 7B (EDS7B). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7B is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A2 are a cause of osteogenesis imperfecta type 1 (OI1). A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 2 (OI2); also known as osteogenesis imperfecta congenita (OIC) or lethal perinatal. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A2 are the cause of Ehlers-Danlos syndrome autosomal recessive cardiac valvular form (EDSCV). A connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. In addition to joint laxity, skin hyperextensibility and friability, and abnormal scar formation, patients have mitral valve prolapse and insufficiency, mitral regurgitation, and aortic insufficiency. Defects in COL1A2 are a cause of osteogenesis imperfecta type 3 (OI3). A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A2 are a cause of osteogenesis imperfecta type 4 (OI4); also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. Belongs to the fibrillar collagen family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type I; extracellular region

Molecular Function: protein binding, bridging; identical protein binding; protein binding; extracellular matrix structural constituent; platelet-derived growth factor binding; metal ion binding; SMAD binding

Biological Process: receptor-mediated endocytosis; blood vessel development; platelet activation; extracellular matrix organization and biogenesis; collagen fibril organization; skin morphogenesis; Rho protein signal transduction; odontogenesis; extracellular matrix disassembly; collagen catabolic process; regulation of blood pressure; transforming growth factor beta receptor signaling pathway; blood coagulation; leukocyte migration; skeletal development

Disease: Ehlers-danlos Syndrome, Type Vii, Autosomal Dominant; Ehlers-danlos Syndrome, Autosomal Recessive, Cardiac Valvular Form; Osteogenesis Imperfecta, Type Ii; Osteogenesis Imperfecta, Type Iii; Osteoporosis; Osteogenesis Imperfecta, Type Iv
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 Research Articles on COL1    1. The novel c.946G>T/p.G316C mutation in COL1A2 gene is associated with with osteogenesis imperfecta type I.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with COL1 elisa kitDiseases associated with COL1 elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Collagen Biosynthesis And Modifying Enzymes Pathway antibodies  Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endothelins Pathway antibodies  Endothelins Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >193 publications with COL1 and Fibrosis
 Cardiovascular Diseases Antibodies  >95 publications with COL1 and Cardiovascular Diseases
 Liver Diseases Antibodies  >43 publications with COL1 and Liver Diseases
 Inflammation Antibodies  >41 publications with COL1 and Inflammation
 Osteoporosis Antibodies  >38 publications with COL1 and Osteoporosis
 Heart Diseases Antibodies  >35 publications with COL1 and Heart Diseases
 Necrosis Antibodies  >32 publications with COL1 and Necrosis
 Liver Cirrhosis Antibodies  >31 publications with COL1 and Liver Cirrhosis
 Brain Diseases Antibodies  >26 publications with COL1 and Brain Diseases
 Kidney Diseases Antibodies  >23 publications with COL1 and Kidney Diseases
Organs/Tissues associated with COL1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >282 publications with COL1 and Bone
 Connective Tissue Antibodies  >202 publications with COL1 and Connective Tissue
 Skin Antibodies  >196 publications with COL1 and Skin
 Muscle Antibodies  >83 publications with COL1 and Muscle
 Blood Antibodies  >74 publications with COL1 and Blood
 Liver Antibodies  >73 publications with COL1 and Liver
 Embryonic Tissue Antibodies  >51 publications with COL1 and Embryonic Tissue
 Vascular Antibodies  >49 publications with COL1 and Vascular
 Lung Antibodies  >46 publications with COL1 and Lung
 Kidney Antibodies  >43 publications with COL1 and Kidney
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