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Col I elisa kit :: Human Collagen Type I , Col I  ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700901
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $835 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
 
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 Product Name   

Collagen Type I , Col I , ELISA Kit

★Popular Item★
 Also Known As   

Human Collagen Type I , Col I  ELISA Kit

 Product Synonym Names    Human Collagen Type I; Col I ELISA Kit; Collagen Type I; Col I
 Product Gene Name   

Col I elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700901 Testing Data    Testing Data PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human Col I. No significant cross-reactivity or interference between human Col I and analogues was observed.
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 Samples    Serum, urine, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    1.5 6 ng/ml -100 ng/ml.
 Sensitivity    The minimum detectable dose of human Col I is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Col I elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Col I purchase    MBS700901 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type I , Col I , ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.5 6 ng/ml -100 ng/ml in biological research samples containing Col I, with an estimated sensitivity of The minimum detectable dose of human Col I is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700901 kit is based on Col I antibody-Col I antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Col I antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Col I. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, urine, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Col I elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ColI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ColI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ColI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ColI bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of Col I elisa kit    Col I elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700901. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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