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COL4a5 elisa kit :: Human Collagen Type IV Alpha 5 (COL4a5) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2090453 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
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 Product Name   

Collagen Type IV Alpha 5 (COL4a5), ELISA Kit

★Popular Item★
 Also Known As   

Collagen Type IV Alpha 5 (COL4a5) ELISA Kit

 Product Gene Name   

COL4a5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of COL4a5. No significant cross-reactivity or interference between COL4a5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    3.12-200ng/mL
 Sensitivity    1.12ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4a5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4a5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL4a5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL4a5 purchase    MBS2090453 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen Type IV Alpha 5 (COL4a5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12-200ng/mL in biological research samples containing COL4a5, with an estimated sensitivity of 1.12ng/mL. The ELISA analytical biochemical technique of the MBS2090453 kit is based on COL4a5 antibody-COL4a5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL4a5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL4a5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL4a5 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4a5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL4a5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4a5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4a5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of COL4a5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for COL4a5 elisa kit    Metabolic pathway; Developmental science; Nutrition metabolism
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 Typical Testing Data/Standard Curve (for reference only) of COL4a5 elisa kit    COL4a5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2090453. Click to request current manual
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 Protein Family    Collagen
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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