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C3a elisa kit :: Human Complement Component 3a ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762839
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Component 3a (C3a), ELISA Kit

★Popular Item★
 Also Known As   

Human Complement Component 3a ELISA Kit

 Product Synonym Names    C3a/C3a-desArg/ASP/Anaphylatoxin/C3
 Product Gene Name   

C3a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS762839 COA    COA PDF
 MBS762839 QC Report    QC Report PDF
 OMIM    605246
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of C3a. No significant cross-reactivity or interference between C3a and analogues was observed.
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 Samples    serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.313-20ng/ml
 Sensitivity    <0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3a were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3a were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C3a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C3a purchase    MBS762839 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 3a (C3a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/ml in biological research samples containing C3a, with an estimated sensitivity of <0.188ng/ml. The ELISA analytical biochemical technique of the MBS762839 kit is based on C3a antibody-C3a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C3a elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C3a can be calculated.
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  Typical Testing Data/Standard Curve (for reference only) of C3a elisa kit    C3a elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762839. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C3a. It may not necessarily be applicable to this product.
 NCBI GI #    30385606
 NCBI GeneID    719
 NCBI Accession #    AAP23198.1 [Other Products]
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 UniProt Secondary Accession #    O43771; Q92868 [Other Products]
 UniProt Related Accession #    Q16581 [Other Products]
 Molecular Weight    53,864 Da
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 NCBI Official Full Name    complement component 3a receptor 1
 NCBI Official Synonym Full Names    complement component 3a receptor 1
 NCBI Official Symbol    C3AR1 [Similar Products]
 NCBI Official Synonym Symbols   
AZ3B; C3AR; HNFAG09
[Similar Products]
 NCBI Protein Information    C3a anaphylatoxin chemotactic receptor
 UniProt Protein Name    C3a anaphylatoxin chemotactic receptor
 UniProt Gene Name    C3AR1 [Similar Products]
 UniProt Synonym Gene Names    AZ3B; C3R1; HNFAG09; C3AR; C3a-R [Similar Products]
 UniProt Entry Name    C3AR_HUMAN
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 UniProt Comments for C3a    C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; integral to plasma membrane; plasma membrane

Molecular Function: C3a anaphylatoxin receptor activity; complement component C3a binding; complement component C3a receptor activity; G-protein coupled receptor activity; phosphoinositide phospholipase C activity

Biological Process: blood circulation; chemotaxis; complement receptor mediated signaling pathway; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; inflammatory response; positive regulation of angiogenesis; positive regulation of smooth muscle cell proliferation; regulation of blood pressure; tolerance induction to nonself antigen
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 Research Articles on C3a    1. Medulloblastoma cells express C3aR, and siRNA-mediated knockdown of C3aR inhibits proliferation of these cells in vitro.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C3a elisa kitPathways associated with C3a elisa kit
 Reference Product  PubMed Publications
 C5AR1 elisa kit  >2 publications with C3a and C5AR1
 FPR1 elisa kit  >1 publications with C3a and FPR1
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
Diseases associated with C3a elisa kitOrgans/Tissues associated with C3a elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with C3a and Inflammation
 Vascular Diseases Antibodies  >4 publications with C3a and Vascular Diseases
 Cardiovascular Diseases Antibodies  >4 publications with C3a and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >4 publications with C3a and Disease Models, Animal
 Arteriosclerosis Antibodies  >3 publications with C3a and Arteriosclerosis
 Atherosclerosis Antibodies  >2 publications with C3a and Atherosclerosis
 Lung Diseases Antibodies  >2 publications with C3a and Lung Diseases
 Kidney Diseases Antibodies  >2 publications with C3a and Kidney Diseases
 Liver Failure Antibodies  >1 publications with C3a and Liver Failure
 Proteinuria Antibodies  >1 publications with C3a and Proteinuria
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with C3a and Blood
 Eye Antibodies  >4 publications with C3a and Eye
 Heart Antibodies  >3 publications with C3a and Heart
 Lung Antibodies  >3 publications with C3a and Lung
 Brain Antibodies  >3 publications with C3a and Brain
 Bone Antibodies  >2 publications with C3a and Bone
 Spleen Antibodies  >2 publications with C3a and Spleen
 Kidney Antibodies  >2 publications with C3a and Kidney
 Uncharacterized Tissue Antibodies  >1 publications with C3a and Uncharacterized Tissue
 Bone Marrow Antibodies  >1 publications with C3a and Bone Marrow
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