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C6 elisa kit :: Human Complement Component 6 (C6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022584 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Component 6 (C6), ELISA Kit

 Also Known As   

Complement Component 6 (C6) ELISA Kit

 Product Gene Name   

C6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    217050
 3D Structure    ModBase 3D Structure for P13671
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Complement Component 6 (C6).
No significant cross-reactivity or interference between Complement Component 6 (C6) and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.625-40ng/mL
 Sensitivity    Typically less than 0.294ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 6 (C6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 6 (C6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C6 purchase    MBS2022584 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 6 (C6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing C6, with an estimated sensitivity of Typically less than 0.294ng/mL. The ELISA analytical biochemical technique of the MBS2022584 kit is based on C6 antibody-C6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for C6 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 6 (C6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 6 (C6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 6 (C6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 6 (C6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of C6 elisa kit    C6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022584. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C6. It may not necessarily be applicable to this product.
 NCBI GI #    38488662
 NCBI GeneID    729
 NCBI Accession #    BAD02321.1 [Other Products]
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 UniProt Primary Accession #    P13671 [Other Products]
 UniProt Related Accession #    P13671 [Other Products]
 Molecular Weight    104,786 Da
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 NCBI Official Full Name    complement component 6
 NCBI Official Synonym Full Names    complement component 6
 NCBI Official Symbol    C6 [Similar Products]
 NCBI Protein Information    complement component C6
 UniProt Protein Name    Complement component C6
 Protein Family    Complement component
 UniProt Gene Name    C6 [Similar Products]
 UniProt Entry Name    CO6_HUMAN
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 NCBI Summary for C6    This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]
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 UniProt Comments for C6    C6: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Molecular Function: protein binding

Biological Process: positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; in utero embryonic development; cytolysis; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 6 Deficiency
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 Research Articles on C6    1. Crystal structure of C5b-6 suggests structural basis for priming assembly of the membrane attack complex.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C6 elisa kitPathways associated with C6 elisa kit
 Reference Product  PubMed Publications
 HIF1A elisa kit  >29 publications with C6 and HIF1A
 PSMA5 elisa kit  >1 publications with C6 and PSMA5
 PSMA3 elisa kit  >1 publications with C6 and PSMA3
 PSMA2 elisa kit  >1 publications with C6 and PSMA2
 PSMA4 elisa kit  >1 publications with C6 and PSMA4
 PSMA6 elisa kit  >1 publications with C6 and PSMA6
 PSMA1 elisa kit  >1 publications with C6 and PSMA1
 Products by Pathway  Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram
Diseases associated with C6 elisa kitOrgans/Tissues associated with C6 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3189 publications with C6 and Nervous System Diseases
 Brain Diseases Antibodies  >1560 publications with C6 and Brain Diseases
 Disease Models, Animal Antibodies  >543 publications with C6 and Disease Models, Animal
 Necrosis Antibodies  >406 publications with C6 and Necrosis
 Immune System Diseases Antibodies  >310 publications with C6 and Immune System Diseases
 Inflammation Antibodies  >289 publications with C6 and Inflammation
 Edema Antibodies  >174 publications with C6 and Edema
 Congenital Abnormalities Antibodies  >150 publications with C6 and Congenital Abnormalities
 Kidney Diseases Antibodies  >140 publications with C6 and Kidney Diseases
 Liver Diseases Antibodies  >122 publications with C6 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >500 publications with C6 and Liver
 Kidney Antibodies  >393 publications with C6 and Kidney
 Heart Antibodies  >319 publications with C6 and Heart
 Lung Antibodies  >262 publications with C6 and Lung
 Connective Tissue Antibodies  >93 publications with C6 and Connective Tissue
 Bladder Antibodies  >77 publications with C6 and Bladder
 Pancreas Antibodies  >38 publications with C6 and Pancreas
 Testis Antibodies  >35 publications with C6 and Testis
 Uterus Antibodies  >16 publications with C6 and Uterus
 Uncharacterized Tissue Antibodies  >1 publications with C6 and Uncharacterized Tissue
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