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CFH elisa kit :: Human Complement Factor H (CFH) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021494 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Complement Factor H (CFH), ELISA Kit

★Popular Item★
 Also Known As   

Complement Factor H (CFH) ELISA Kit

 Product Synonym Names    CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
 Product Gene Name   

CFH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2021494 COA    COA PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH).
No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    4.7-300ng/mL
 Sensitivity    < 2.1ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CFH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFH purchase    MBS2021494 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Factor H (CFH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 4.7-300ng/mL in biological research samples containing CFH, with an estimated sensitivity of < 2.1ng/mL. The ELISA analytical biochemical technique of the MBS2021494 kit is based on CFH antibody-CFH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CFH elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H (CFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H (CFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H (CFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CFH elisa kit    Metabolic pathway; Infection immunity; Immune molecule
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 Typical Testing Data/Standard Curve (for reference only) of CFH elisa kit    CFH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021494. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CFH. It may not necessarily be applicable to this product.
 NCBI GI #    576647241
 NCBI GeneID    3075
 NCBI Accession #    AHH32464.1 [Other Products]
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 UniProt Related Accession #    P08603 [Other Products]
 Molecular Weight    6,603 Da
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 NCBI Official Full Name    complement factor H, partial
 NCBI Official Synonym Full Names    complement factor H
 NCBI Official Symbol    CFH [Similar Products]
 NCBI Official Synonym Symbols   
FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
[Similar Products]
 NCBI Protein Information    complement factor H
 UniProt Protein Name    Complement factor H
 UniProt Synonym Protein Names   
Complement factor H
 Protein Family    Complement factor
 UniProt Entry Name    W5U1X0_HUMAN
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 NCBI Summary for CFH    This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
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 UniProt Comments for CFH    CFH: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway. Genetic variations in CFH are associated with basal laminar drusen (BLD); also known as drusen of Bruch membrane or cuticular drusen or grouped early adult-onset drusen. Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch membrane. Basal laminar drusen refers to an early adult-onset drusen phenotype that shows a pattern of uniform small, slightly raised yellow subretinal nodules randomly scattered in the macula. In later stages, these drusen often become more numerous, with clustered groups of drusen scattered throughout the retina. In time these small basal laminar drusen may expand and ultimately lead to a serous pigment epithelial detachment of the macula that may result in vision loss. Defects in CFH are the cause of complement factor H deficiency (CFHD). A disorder that can manifest as several different phenotypes, including asymptomatic, recurrent bacterial infections, and renal failure. Laboratory features usually include decreased serum levels of factor H, complement component C3, and a decrease in other terminal complement components, indicating activation of the alternative complement pathway. It is associated with a number of renal diseases with variable clinical presentation and progression, including membranoproliferative glomerulonephritis and atypical hemolytic uremic syndrome. Defects in CFH are a cause of susceptibility to hemolytic uremic syndrome atypical type 1 (AHUS1). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding

Biological Process: complement activation, alternative pathway; regulation of complement activation; innate immune response; complement activation

Disease: Complement Factor H Deficiency; Basal Laminar Drusen; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 1; Macular Degeneration, Age-related, 4
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 Research Articles on CFH    1. Patients carrying 4 risk alleles in CFH and ARMS2 developed neovascular AMD 12.2 (95% CI, 6.2-18.3) years earlier than patients with zero risk alleles (P < .001).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CFH elisa kitPathways associated with CFH elisa kit
 Reference Product  PubMed Publications
 ARMS2 elisa kit  >215 publications with CFH and ARMS2
 HTRA1 elisa kit  >139 publications with CFH and HTRA1
 CFI elisa kit  >68 publications with CFH and CFI
 PLEKHA1 elisa kit  >14 publications with CFH and PLEKHA1
 ITGAM elisa kit  >4 publications with CFH and ITGAM
 ADM elisa kit  >1 publications with CFH and ADM
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Regulation Of Complement Cascade Pathway antibodies  Regulation Of Complement Cascade Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
Diseases associated with CFH elisa kitOrgans/Tissues associated with CFH elisa kit
 Disease Name  Pubmed Publications
 Macular Degeneration Antibodies  >540 publications with CFH and Macular Degeneration
 Macular Degeneration, Age-Related, 4 Antibodies  >205 publications with CFH and Macular Degeneration, Age-Related, 4
 Kidney Diseases Antibodies  >174 publications with CFH and Kidney Diseases
 Atypical Hemolytic Uremic Syndrome Antibodies  >141 publications with CFH and Atypical Hemolytic Uremic Syndrome
 Inflammation Antibodies  >94 publications with CFH and Inflammation
 Cardiovascular Diseases Antibodies  >68 publications with CFH and Cardiovascular Diseases
 Atrophy Antibodies  >58 publications with CFH and Atrophy
 Glomerulonephritis, Membranoproliferative Antibodies  >43 publications with CFH and Glomerulonephritis, Membranoproliferative
 Hypertension Antibodies  >40 publications with CFH and Hypertension
 Heart Diseases Antibodies  >27 publications with CFH and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >289 publications with CFH and Eye
 Kidney Antibodies  >133 publications with CFH and Kidney
 Vascular Antibodies  >95 publications with CFH and Vascular
 Ear Antibodies  >45 publications with CFH and Ear
 Brain Antibodies  >38 publications with CFH and Brain
 Liver Antibodies  >34 publications with CFH and Liver
 Heart Antibodies  >27 publications with CFH and Heart
 Skin Antibodies  >14 publications with CFH and Skin
 Lung Antibodies  >14 publications with CFH and Lung
 Nerve Antibodies  >13 publications with CFH and Nerve
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