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C4b elisa kit :: Human Complement fragment 4b, C4b ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700316
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

Complement fragment 4b, C4b, ELISA Kit

★Popular Item★
 Also Known As   

Human Complement fragment 4b, C4b ELISA Kit

 Product Synonym Names    Human Complement fragment 4b; C4b ELISA Kit; Complement fragment 4b; C4b
 Product Gene Name   

C4b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700316 Testing Data    Testing Data PDF
 OMIM    614379
 3D Structure    ModBase 3D Structure for P0C0L5
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human C4b. No significant cross-reactivity or interference between human C4b and analogues was observed.
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 Samples    Serum, plasma
 Assay Type    Sandwich
 Detection Range    15.6 ng/ml -1000 ng/ml.
 Sensitivity    The minimum detectable dose of human C4b is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of C4b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for C4b purchase    MBS700316 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement fragment 4b, C4b, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 ng/ml -1000 ng/ml in biological research samples containing C4b, with an estimated sensitivity of The minimum detectable dose of human C4b is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS700316 kit is based on C4b antibody-C4b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C4b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C4b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for C4b elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4b bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of C4b elisa kit    C4b elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700316. Click to request current manual
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NCBI/Uniprot data below describe general gene information for C4b. It may not necessarily be applicable to this product.
 NCBI GI #    178557739
 NCBI GeneID    721
 NCBI Accession #    NP_001002029.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001002029.3 [Other Products]
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 UniProt Primary Accession #    P0C0L5 [Other Products]
 UniProt Secondary Accession #    P01028; P78445; Q13160; Q13906; Q14033; Q14835; Q6U2E9; Q6U2G1; Q6U2I5; Q6U2L1; A2BHY4 [Other Products]
 UniProt Related Accession #    P0C0L5 [Other Products]
 Molecular Weight    192,751 Da
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 NCBI Official Full Name    complement C4-B preproprotein
 NCBI Official Synonym Full Names    complement component 4B (Chido blood group)
 NCBI Official Symbol    C4B [Similar Products]
 NCBI Official Synonym Symbols   
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
[Similar Products]
 NCBI Protein Information    complement C4-B; Chido form of C4; complement C4B1a; basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
 UniProt Protein Name    Complement C4-B
 UniProt Synonym Protein Names   
Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3Cleaved into the following 6 chains:Complement C4 beta chain; Complement C4-B alpha chain; C4a anaphylatoxin; C4b-B; C4d-B; Complement C4 gamma chain
 Protein Family    Complement C4
 UniProt Gene Name    C4B [Similar Products]
 UniProt Synonym Gene Names    CO4; CPAMD3
C4B_2
[Similar Products]
 UniProt Entry Name    CO4B_HUMAN
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 NCBI Summary for C4b    This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq, Jul 2008]
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 UniProt Comments for C4b    Function: Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.2 PublicationsManual assertion based on experiment in:Ref.16
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 Research Articles on C4b    1. Report clinical/genomic significance of donor-specific antibody-positive/C4d-negative and donor-specific antibody-negative/C4d-negative transplant glomerulopathy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with C4b elisa kitPathways associated with C4b elisa kit
 Reference Product  PubMed Publications
 C1S elisa kit  >65 publications with C4b and C1S
 TNXA elisa kit  >15 publications with C4b and TNXA
 CYP21A2 elisa kit  >12 publications with C4b and CYP21A2
 HLA-ABC elisa kit  >3 publications with C4b and HLA-ABC
 C5AR1 elisa kit  >1 publications with C4b and C5AR1
 Products by Pathway  Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Staphylococcus Aureus Infection Pathway antibodies  Staphylococcus Aureus Infection Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
 Systemic Lupus Erythematosus Pathway antibodies  Systemic Lupus Erythematosus Pathway Diagram
Diseases associated with C4b elisa kitOrgans/Tissues associated with C4b elisa kit
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >331 publications with C4b and Autoimmune Diseases
 Vascular Diseases Antibodies  >189 publications with C4b and Vascular Diseases
 Kidney Diseases Antibodies  >151 publications with C4b and Kidney Diseases
 Inflammation Antibodies  >149 publications with C4b and Inflammation
 Thrombosis Antibodies  >106 publications with C4b and Thrombosis
 Nervous System Diseases Antibodies  >94 publications with C4b and Nervous System Diseases
 Neoplasms Antibodies  >87 publications with C4b and Neoplasms
 Necrosis Antibodies  >80 publications with C4b and Necrosis
 Arthritis, Rheumatoid Antibodies  >73 publications with C4b and Arthritis, Rheumatoid
 Joint Diseases Antibodies  >67 publications with C4b and Joint Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1040 publications with C4b and Blood
 Kidney Antibodies  >309 publications with C4b and Kidney
 Liver Antibodies  >125 publications with C4b and Liver
 Heart Antibodies  >79 publications with C4b and Heart
 Muscle Antibodies  >45 publications with C4b and Muscle
 Lung Antibodies  >44 publications with C4b and Lung
 Skin Antibodies  >43 publications with C4b and Skin
 Brain Antibodies  >43 publications with C4b and Brain
 Ovary Antibodies  >16 publications with C4b and Ovary
 Intestine Antibodies  >16 publications with C4b and Intestine
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