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CKMB elisa kit :: Human Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265355
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Creatine Kinase MB Isoenzyme (CKMB), ELISA Kit

★Popular Item★
 Also Known As   

Human Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit

 Product Gene Name   

CKMB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    123310
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant CKMB concentration.
 Assay Type    Sandwich
 Detection Range    100 ng/ml-1.56 ng/ml
 Sensitivity    0.5 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CKMB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CKMB purchase    MBS265355 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine Kinase MB Isoenzyme (CKMB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100 ng/ml-1.56 ng/ml in biological research samples containing CKMB, with an estimated sensitivity of 0.5 ng/ml. The ELISA analytical biochemical technique of the MBS265355 kit is based on CKMB antibody-CKMB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKMB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKMB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant CKMB concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CKMB elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CKMB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve of CKMB elisa kit    CKMB elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS265355. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CKMB. It may not necessarily be applicable to this product.
 NCBI GI #    21536288
 NCBI GeneID    1158
 NCBI Accession #    NP_001815.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001824.4 [Other Products]
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 UniProt Secondary Accession #    Q96QL9 [Other Products]
 UniProt Related Accession #    P06732 [Other Products]
 Molecular Weight    43,101 Da
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 NCBI Official Full Name    creatine kinase M-type
 NCBI Official Synonym Full Names    creatine kinase, muscle
 NCBI Official Symbol    CKM [Similar Products]
 NCBI Official Synonym Symbols   
CKMM; M-CK
[Similar Products]
 NCBI Protein Information    creatine kinase M-type; creatine kinase M chain
 UniProt Protein Name    Creatine kinase M-type
 UniProt Synonym Protein Names   
Creatine kinase M chain; M-CKCleaved into the following chain:Creatine kinase M-type, N-terminally processed
 UniProt Gene Name    CKM [Similar Products]
 UniProt Synonym Gene Names    CKMM [Similar Products]
 UniProt Entry Name    KCRM_HUMAN
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 NCBI Summary for CKMB    The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CKMB    CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation
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 Research Articles on CKMB    1. Estimation of CK and its CK isoenzyme fractions can aid in quick and accurate diagnosis of tubal ectopic pregnancy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CKMB elisa kitPathways associated with CKMB elisa kit
 Reference Product  PubMed Publications
 CKB elisa kit  >14 publications with CKMB and CKB
 ATP2A1 elisa kit  >2 publications with CKMB and ATP2A1
 TNNC2 elisa kit  >2 publications with CKMB and TNNC2
 AK1 elisa kit  >1 publications with CKMB and AK1
 MYLPF elisa kit  >1 publications with CKMB and MYLPF
 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Creatine Metabolism Pathway antibodies  Creatine Metabolism Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram
Diseases associated with CKMB elisa kitOrgans/Tissues associated with CKMB elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >12 publications with CKMB and Nervous System Diseases
 Heart Diseases Antibodies  >11 publications with CKMB and Heart Diseases
 Myocardial Infarction Antibodies  >7 publications with CKMB and Myocardial Infarction
 Kidney Diseases Antibodies  >5 publications with CKMB and Kidney Diseases
 Lung Neoplasms Antibodies  >4 publications with CKMB and Lung Neoplasms
 Ischemia Antibodies  >4 publications with CKMB and Ischemia
 Brain Diseases Antibodies  >3 publications with CKMB and Brain Diseases
 Liver Diseases Antibodies  >3 publications with CKMB and Liver Diseases
 Cardiomyopathies Antibodies  >3 publications with CKMB and Cardiomyopathies
 Weight Loss Antibodies  >2 publications with CKMB and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >53 publications with CKMB and Muscle
 Heart Antibodies  >26 publications with CKMB and Heart
 Brain Antibodies  >8 publications with CKMB and Brain
 Kidney Antibodies  >8 publications with CKMB and Kidney
 Lung Antibodies  >6 publications with CKMB and Lung
 Liver Antibodies  >6 publications with CKMB and Liver
 Embryonic Tissue Antibodies  >3 publications with CKMB and Embryonic Tissue
 Bone Antibodies  >3 publications with CKMB and Bone
 Vascular Antibodies  >3 publications with CKMB and Vascular
 Nerve Antibodies  >2 publications with CKMB and Nerve
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