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| Product Name
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Creatine Kinase MB (CK-MB), ELISA Kit ★Popular Item★
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| Also Known As
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Human Creatine Kinase MB ELISA Kit
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| Product Synonym Names
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CKMB (Creatine Kinase MB Isoenzyme)/CK-MB
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS761783 COA
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of CK-MB . No significant cross-reactivity or interference between CK-MB and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates and other biological fluids
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| Assay Type
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Sandwich
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| Detection Range
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0.156-10ng/ml
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| Sensitivity
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<0.094ng/ml
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CKMB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CKMB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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| Preparation and Storage
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Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of CK-MB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for CK-MB purchase
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MBS761783 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine Kinase MB (CK-MB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing CK-MB, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS761783 kit is based on CK-MB antibody-CK-MB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-MB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK-MB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CK-MB elisa kit
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Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CK-MB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CK-MB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-MB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK-MB can be calculated.
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| Typical Testing Data/Standard Curve (for reference only) of CK-MB elisa kit
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| Sample Manual Insert of MBS761783. Click to request current manual
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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ELISA Kit 
