|
| Product Name
|
|
Cubilin (CUBN), ELISA Kit
|
| Also Known As
|
|
Cubilin (CUBN) ELISA Kit
|
| Product Synonym Names
|
|
IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Manual Insert
|
|
 
|
|
| 3D Structure
|
|
ModBase 3D Structure for O70244
|
| Species Reactivity
|
|
Human
|
|
| Specificity
|
|
This assay has high sensitivity and excellent specificity for detection of Cubilin (CUBN).
No significant cross-reactivity or interference between Cubilin (CUBN) and analogues was observed.
|
|
|
| Samples
|
|
Tissue homogenates and other biological fluids
|
| Assay Type
|
|
Sandwich
|
| Detection Range
|
|
0.781-50ng/mL
|
| Sensitivity
|
|
Typically less than 0.34ng/mL.
|
| Precision
|
|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cubilin (CUBN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cubilin (CUBN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
|
| Assay Procedure Summary
|
|
1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
|
|
|
| Preparation and Storage
|
|
For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.
For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|
| Product Note
|
|
Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
|
| Other Notes
|
|
Small volumes of CUBN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for CUBN purchase
|
|
MBS2021105 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cubilin (CUBN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing CUBN, with an estimated sensitivity of Typically less than 0.34ng/mL. The ELISA analytical biochemical technique of the MBS2021105 kit is based on CUBN antibody-CUBN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CUBN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CUBN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
Product Description specifically for CUBN elisa kit
|
|
Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cubilin (CUBN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cubilin (CUBN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
|
|
|
|
| Typical Testing Data/Standard Curve (for reference only) of CUBN elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS2021105 |
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for CUBN. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
16758040
|
| NCBI GeneID
|
|
80848
|
| NCBI Accession #
|
|
NP_445784.1
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_053332.2
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
O70244
|
[Other Products]
|
| UniProt Related Accession #
|
|
O70244
|
[Other Products]
|
| Molecular Weight
|
|
398,987 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
cubilin
|
| NCBI Official Synonym Full Names
|
|
cubilin (intrinsic factor-cobalamin receptor)
|
| NCBI Official Symbol
|
|
Cubn
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
IFCR; MGA1; GP280
|
[Similar Products]
|
| NCBI Protein Information
|
|
cubilin
|
| UniProt Protein Name
|
|
Cubilin
|
| UniProt Synonym Protein Names
|
|
460 kDa receptor; Glycoprotein 280; gp280; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor
|
| Protein Family
|
|
Cubilin
|
| UniProt Gene Name
|
|
Cubn
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
Ifcr; gp280
|
[Similar Products]
|
| UniProt Entry Name
|
|
CUBN_RAT
|
|
|
| NCBI Summary for CUBN
|
|
receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]
|
|
|
| UniProt Comments for CUBN
|
|
CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.
Cellular Component: Golgi apparatus; lysosomal lumen; protein complex; lysosomal membrane; endoplasmic reticulum; brush border membrane; coated pit; membrane; apical part of cell; endocytic vesicle; cytoplasm; apical plasma membrane; endosome membrane; brush border; endosome
Molecular Function: identical protein binding; protein homodimerization activity; hemoglobin binding; cobalamin transporter activity; calcium ion binding; receptor activity; cobalamin binding
Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; vitamin metabolic process; in utero embryonic development; lipoprotein transport; cobalamin transport; hemoglobin import; response to nutrient
|
|
TOP
|
|
| Research Articles on CUBN
|
|
1. We conclude that the megalin/cubilin/amnionless/Dab2 complex/es participate in intestinal processes, mainly during the lactation period and that Dab2 may act as an adaptor in intestinal processes mediated by ApoER2 and VLDLR.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with CUBN elisa kit | Pathways associated with CUBN elisa kit |
|
|
|
| Diseases associated with CUBN elisa kit | Organs/Tissues associated with CUBN elisa kit |
|
|
|
|
|
|
TOP
|
ELISA Kit 
