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CSTB elisa kit :: Human Cystatin B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS761966
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
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 Product Name   

Cystatin B (CSTB), ELISA Kit

★Popular Item★
 Also Known As   

Human Cystatin B ELISA Kit

 Product Synonym Names    Cystatin B/CSTB/Stefin-B/CPI-B/PME/EPM1/Liver thiol proteinase inhibitor
 Product Gene Name   

CSTB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS761966 COA    COA PDF
 OMIM    254800
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CSTB. No significant cross-reactivity or interference between CSTB and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CSTB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CSTB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CSTB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CSTB purchase    MBS761966 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystatin B (CSTB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing CSTB, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS761966 kit is based on CSTB antibody-CSTB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSTB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSTB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CSTB elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CSTB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CSTB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CSTB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CSTB can be calculated.
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 Typical Standard Curve/Testing Data of CSTB elisa kit    CSTB elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS761966. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.
 NCBI GI #    7263012
 NCBI Accession #    AAF44059.1 [Other Products]
 Molecular Weight    11,140 Da
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 NCBI Official Full Name    cystatin B
 UniProt Protein Name    Cystatin-B
 UniProt Synonym Protein Names   
CPI-B; Liver thiol proteinase inhibitor; Stefin-B
 Protein Family    Cystatin
 UniProt Gene Name    CSTB [Similar Products]
 UniProt Synonym Gene Names    CST6; STFB [Similar Products]
 UniProt Entry Name    CYTB_HUMAN
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 UniProt Comments for CSTB    CSTB: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1). EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. Belongs to the cystatin family.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; extracellular space; nucleolus

Molecular Function: cysteine protease inhibitor activity; endopeptidase inhibitor activity; protease binding

Biological Process: adult locomotory behavior; negative regulation of peptidase activity; negative regulation of proteolysis

Disease: Myoclonic Epilepsy Of Unverricht And Lundborg
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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