Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow CST2 rightarrow Human CST2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Pepsinogen I (PGA3) Protein
 • ku86 ELISA Kit
 • CS ELISA Kit
 • HIV-1 gp41 Recombinant Protein
 • Thyroxine Antibody
 • Plaque-size-host range protein (PS-HR) Recombinant Protein
 • Beta-Endorphin, beta-EP ELISA Kit (beta-EP) ELISA Kit
 • CD31 Antibody
 • PLBD2 Antibody
 • HTR1E - 5-HT1E Receptor Antibody
 • Mineralocorticoid Receptor (NR3C2) Antibody
 • ACYP1 Peptide
 • Estriol 6 Antibody
 • Prostate Specific Antigen (PSA) (KLK3) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

CST2 elisa kit :: Human Cystatin SA (CST2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7211145
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cystatin SA (CST2), ELISA Kit

★Popular Item★
 Also Known As   

Human Cystatin SA (CST2) ELISA Kit

 Product Gene Name   

CST2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    123856
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of CST2. No significant cross-reactivity or interference between CST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CST2 and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CST2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CST2 purchase    MBS7211145 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystatin SA (CST2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing CST2, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7211145 kit is based on CST2 antibody-CST2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CST2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CST2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for CST2 elisa kit

   Intended Uses: This CST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CST2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CST2 antibody and an CST2-HRP conjugate. The assay sample and buffer are incubated together with CST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CST2 concentration since CST2 from samples and CST2-HRP conjugate compete for the anti-CST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CST2 from the sample, fewer sites are left to bind CST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CST2 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for CST2 elisa kit    Cell Biology
Section Bar
 Typical Standard Curve/Testing Data of CST2 elisa kit    CST2 elisa kit Typical Standard Curve/Testing Data image
Table BarTOPTable Bar
Sample Manual Insert of MBS7211145. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CST2. It may not necessarily be applicable to this product.
 NCBI GI #    4503105
 NCBI GeneID    1470
 NCBI Accession #    NP_001313.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001322.2 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q9UCQ7 [Other Products]
 UniProt Related Accession #    P09228 [Other Products]
 Molecular Weight    16,445 Da
Table BarTOPTable Bar
 NCBI Official Full Name    cystatin-SA
 NCBI Official Synonym Full Names    cystatin SA
 NCBI Official Symbol    CST2 [Similar Products]
 NCBI Protein Information    cystatin-SA; cystatin 2; cystatin-2; cystatin S5; cystatin-S5; cysteine-proteinase inhibitor; salivary cysteine (thiol) protease inhibitor
 UniProt Protein Name    Cystatin-SA
 UniProt Synonym Protein Names   
Cystatin-2; Cystatin-S5
 Protein Family    Cystatin
 UniProt Gene Name    CST2 [Similar Products]
 UniProt Entry Name    CYTT_HUMAN
Section Bar
 NCBI Summary for CST2    The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CST2    CST2: Thiol protease inhibitor. Belongs to the cystatin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste
Table BarTOPTable Bar
 Research Articles on CST2    1. This data suggests an autoimmune mechanism behind chronic mucocutaneous candidiasis in autoimmune polyendocrine syndrome type I and provides rationale for evaluating cystatin SA1 in antifungal therapy.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with CST2 elisa kitOrgans/Tissues associated with CST2 elisa kit
 Products by Pathway  Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Salivary Gland Antibodies  >6 publications with CST2 and Salivary Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions