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CYP2E1 elisa kit :: Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021746 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytochrome P450 2E1 (CYP2E1), ELISA Kit

★Popular Item★
 Also Known As   

Cytochrome P450 2E1 (CYP2E1) ELISA Kit

 Product Synonym Names    CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1
 Product Gene Name   

CYP2E1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    124040
 3D Structure    ModBase 3D Structure for P05181
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2E1 (CYP2E1).
No significant cross-reactivity or interference between Cytochrome P450 2E1 (CYP2E1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.78-50ng/mL
 Sensitivity    < 0.32ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Cytochrome P450 2E1 (CYP2E1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Cytochrome P450 2E1 (CYP2E1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CYP2E1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CYP2E1 purchase    MBS2021746 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 2E1 (CYP2E1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50ng/mL in biological research samples containing CYP2E1, with an estimated sensitivity of < 0.32ng/mL. The ELISA analytical biochemical technique of the MBS2021746 kit is based on CYP2E1 antibody-CYP2E1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP2E1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP2E1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CYP2E1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1 (CYP2E1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2E1 (CYP2E1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2E1 (CYP2E1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2E1 (CYP2E1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CYP2E1 elisa kit    Signal transduction; Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of CYP2E1 elisa kit    CYP2E1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021746. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CYP2E1. It may not necessarily be applicable to this product.
 NCBI GI #    10834998
 NCBI GeneID    1571
 NCBI Accession #    NP_000764.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000773.3 [Other Products]
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 UniProt Primary Accession #    P05181 [Other Products]
 UniProt Secondary Accession #    Q5VZD5; Q6NWT9; Q9UK47 [Other Products]
 UniProt Related Accession #    P05181 [Other Products]
 Molecular Weight    56,849 Da
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 NCBI Official Full Name    cytochrome P450 2E1
 NCBI Official Synonym Full Names    cytochrome P450, family 2, subfamily E, polypeptide 1
 NCBI Official Symbol    CYP2E1 [Similar Products]
 NCBI Official Synonym Symbols   
CPE1; CYP2E; P450-J; P450C2E
[Similar Products]
 NCBI Protein Information    cytochrome P450 2E1
 UniProt Protein Name    Cytochrome P450 2E1
 UniProt Synonym Protein Names   
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-J
 Protein Family    Cytochrome
 UniProt Gene Name    CYP2E1 [Similar Products]
 UniProt Synonym Gene Names    CYP2E [Similar Products]
 UniProt Entry Name    CP2E1_HUMAN
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 NCBI Summary for CYP2E1    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CYP2E1    CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - arachidonic acid; Mitochondrial; Endoplasmic reticulum; Oxidoreductase; EC 1.14.13.n7; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: intrinsic to endoplasmic reticulum membrane; Golgi membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; response to ethanol; triacylglycerol metabolic process; response to ozone; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; response to organic nitrogen; drug metabolic process
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 Research Articles on CYP2E1    1. study is the first to our knowledge to show that polymorphisms in the rs1004467 SNP of CYP17A1 and rs3813865 SNP of CYP2E1 correlate with susceptibility to HAPC
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CYP2E1 elisa kitPathways associated with CYP2E1 elisa kit
 Reference Product  PubMed Publications
 GSTM1 elisa kit  >265 publications with CYP2E1 and GSTM1
 ALDH2 elisa kit  >138 publications with CYP2E1 and ALDH2
 EPHX1 elisa kit  >117 publications with CYP2E1 and EPHX1
 PTGS2 elisa kit  >37 publications with CYP2E1 and PTGS2
 ADH1B elisa kit  >35 publications with CYP2E1 and ADH1B
 GSTA4 elisa kit  >12 publications with CYP2E1 and GSTA4
 GSTA1 elisa kit  >8 publications with CYP2E1 and GSTA1
 GSTA2 elisa kit  >6 publications with CYP2E1 and GSTA2
 GSTA3 elisa kit  >1 publications with CYP2E1 and GSTA3
 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 CYP2E1 Reactions Pathway antibodies  CYP2E1 Reactions Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway antibodies  Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway Diagram
 Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway antibodies  Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway Diagram
 Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway antibodies  Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway Diagram
Diseases associated with CYP2E1 elisa kitOrgans/Tissues associated with CYP2E1 elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >951 publications with CYP2E1 and Liver Diseases
 Drug-Induced Liver Injury Antibodies  >456 publications with CYP2E1 and Drug-Induced Liver Injury
 Necrosis Antibodies  >344 publications with CYP2E1 and Necrosis
 Fatty Liver Antibodies  >337 publications with CYP2E1 and Fatty Liver
 Drug-Related Side Effects and Adverse Reactions Antibodies  >282 publications with CYP2E1 and Drug-Related Side Effects and Adverse Reactions
 Liver Neoplasms Antibodies  >226 publications with CYP2E1 and Liver Neoplasms
 Alcoholism Antibodies  >216 publications with CYP2E1 and Alcoholism
 Inflammation Antibodies  >209 publications with CYP2E1 and Inflammation
 Hepatitis Antibodies  >178 publications with CYP2E1 and Hepatitis
 Adenocarcinoma Antibodies  >157 publications with CYP2E1 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3206 publications with CYP2E1 and Liver
 Blood Antibodies  >956 publications with CYP2E1 and Blood
 Lung Antibodies  >328 publications with CYP2E1 and Lung
 Brain Antibodies  >155 publications with CYP2E1 and Brain
 Muscle Antibodies  >114 publications with CYP2E1 and Muscle
 Bone Antibodies  >47 publications with CYP2E1 and Bone
 Skin Antibodies  >34 publications with CYP2E1 and Skin
 Testis Antibodies  >25 publications with CYP2E1 and Testis
 Prostate Antibodies  >20 publications with CYP2E1 and Prostate
 Ovary Antibodies  >15 publications with CYP2E1 and Ovary
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