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CTLA4 elisa kit :: Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021221 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $475 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,590 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,670 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), ELISA Kit

★Popular Item★
 Also Known As   

Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

 Product Synonym Names    CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4
 Product Gene Name   

CTLA4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    109100
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4).
No significant cross-reactivity or interference between Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    31.2-2,000pg/mL
 Sensitivity    < 12.4pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CTLA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CTLA4 purchase    MBS2021221 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2,000pg/mL in biological research samples containing CTLA4, with an estimated sensitivity of < 12.4pg/mL. The ELISA analytical biochemical technique of the MBS2021221 kit is based on CTLA4 antibody-CTLA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CTLA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CTLA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CTLA4 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for CTLA4 elisa kit    Signal transduction; Immune molecule; Autoimmunity
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 Typical Testing Data/Standard Curve (for reference only) of CTLA4 elisa kit    CTLA4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021221. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CTLA4. It may not necessarily be applicable to this product.
 NCBI GI #    27735177
 NCBI GeneID    1493
 NCBI Accession #    P16410.3 [Other Products]
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 UniProt Secondary Accession #    O95653; Q0PP65; Q52MC1; Q53TD5; Q5S005; Q8WXJ1; Q96P43; Q9UKN9; A0N1S0; E9PDH0 [Other Products]
 UniProt Related Accession #    P16410 [Other Products]
 Molecular Weight    19,145 Da
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 NCBI Official Full Name    Cytotoxic T-lymphocyte protein 4
 NCBI Official Synonym Full Names    cytotoxic T-lymphocyte-associated protein 4
 NCBI Official Symbol    CTLA4 [Similar Products]
 NCBI Official Synonym Symbols   
CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
[Similar Products]
 NCBI Protein Information    cytotoxic T-lymphocyte protein 4
 UniProt Protein Name    Cytotoxic T-lymphocyte protein 4
 UniProt Synonym Protein Names   
Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD_antigen: CD152
 Protein Family    Cytotoxic T-lymphocyte protein
 UniProt Gene Name    CTLA4 [Similar Products]
 UniProt Synonym Gene Names    CD152; CTLA-4 [Similar Products]
 UniProt Entry Name    CTLA4_HUMAN
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 NCBI Summary for CTLA4    This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CTLA4    CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Molecular Function: protein binding

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of apoptosis; negative regulation of regulatory T cell differentiation; negative regulation of immune response; T cell costimulation; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus

Disease: Autoimmune Lymphoproliferative Syndrome, Type V; Celiac Disease, Susceptibility To, 3; Diabetes Mellitus, Insulin-dependent, 12; Hashimoto Thyroiditis; Systemic Lupus Erythematosus
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 Research Articles on CTLA4    1. A possible additive combined effect between CD40-1C>T and CTLA4+6230G>A polymorphisms in the development of Graves' disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CTLA4 elisa kitPathways associated with CTLA4 elisa kit
 Reference Product  PubMed Publications
 CD80 elisa kit  >1017 publications with CTLA4 and CD80
 CD86 elisa kit  >655 publications with CTLA4 and CD86
 FOXP3 elisa kit  >619 publications with CTLA4 and FOXP3
 LCK elisa kit  >18 publications with CTLA4 and LCK
 PTPN11 elisa kit  >13 publications with CTLA4 and PTPN11
 CD276 elisa kit  >10 publications with CTLA4 and CD276
 FYN elisa kit  >7 publications with CTLA4 and FYN
 LYN elisa kit  >5 publications with CTLA4 and LYN
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
Diseases associated with CTLA4 elisa kitOrgans/Tissues associated with CTLA4 elisa kit
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1633 publications with CTLA4 and Immune System Diseases
 Melanoma Antibodies  >538 publications with CTLA4 and Melanoma
 Inflammation Antibodies  >378 publications with CTLA4 and Inflammation
 Necrosis Antibodies  >373 publications with CTLA4 and Necrosis
 Arthritis, Rheumatoid Antibodies  >228 publications with CTLA4 and Arthritis, Rheumatoid
 Carcinoma Antibodies  >199 publications with CTLA4 and Carcinoma
 Graves Disease Antibodies  >179 publications with CTLA4 and Graves Disease
 Liver Diseases Antibodies  >157 publications with CTLA4 and Liver Diseases
 Neoplasms, Experimental Antibodies  >157 publications with CTLA4 and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >92 publications with CTLA4 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3179 publications with CTLA4 and Blood
 Lymph Node Antibodies  >247 publications with CTLA4 and Lymph Node
 Lung Antibodies  >213 publications with CTLA4 and Lung
 Kidney Antibodies  >206 publications with CTLA4 and Kidney
 Prostate Antibodies  >87 publications with CTLA4 and Prostate
 Mammary Gland Antibodies  >1 publications with CTLA4 and Mammary Gland
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