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PHGDH elisa kit :: Human D-3-phosphoglycerate dehydrogenase, PHGDH ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2602726
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

D-3-phosphoglycerate dehydrogenase, PHGDH, ELISA Kit

★Popular Item★
 Also Known As   

Human D-3-phosphoglycerate dehydrogenase, PHGDH ELISA Kit

 Product Gene Name   

PHGDH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606879
 3D Structure    ModBase 3D Structure for O43175
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    Up to 0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PHGDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PHGDH purchase    MBS2602726 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the D-3-phosphoglycerate dehydrogenase, PHGDH, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing PHGDH, with an estimated sensitivity of Up to 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS2602726 kit is based on PHGDH antibody-PHGDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PHGDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PHGDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PHGDH elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PHGDH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of PHGDH elisa kit    PHGDH elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2602726. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PHGDH. It may not necessarily be applicable to this product.
 NCBI GI #    23308577
 NCBI GeneID    26227
 NCBI Accession #    NP_006614.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006623.3 [Other Products]
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 UniProt Primary Accession #    O43175 [Other Products]
 UniProt Secondary Accession #    Q5SZU3; Q9BQ01; B2RD08 [Other Products]
 UniProt Related Accession #    O43175 [Other Products]
 Molecular Weight    56,651 Da
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 NCBI Official Full Name    D-3-phosphoglycerate dehydrogenase
 NCBI Official Synonym Full Names    phosphoglycerate dehydrogenase
 NCBI Official Symbol    PHGDH [Similar Products]
 NCBI Official Synonym Symbols   
NLS; PDG; PGD; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113
[Similar Products]
 NCBI Protein Information    D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113
 UniProt Protein Name    D-3-phosphoglycerate dehydrogenase
 UniProt Gene Name    PHGDH [Similar Products]
 UniProt Synonym Gene Names    PGDH3; 3-PGDH [Similar Products]
 UniProt Entry Name    SERA_HUMAN
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 NCBI Summary for PHGDH    This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
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 UniProt Comments for PHGDH    PHGDH: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency). It is characterized by congenital microcephaly, psychomotor retardation, and seizures. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 1.1.1.95; Oxidoreductase; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: cytosol

Molecular Function: electron carrier activity; phosphoglycerate dehydrogenase activity; NAD binding

Biological Process: glial cell development; neural tube development; glycine metabolic process; gamma-aminobutyric acid metabolic process; glutamine metabolic process; L-serine biosynthetic process; spinal cord development; regulation of gene expression; amino acid biosynthetic process; brain development; taurine metabolic process; neurite development; threonine metabolic process

Disease: Phosphoglycerate Dehydrogenase Deficiency; Neu-laxova Syndrome 1
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 Research Articles on PHGDH    1. We report on the identification of homozygous mutations in PHGDH and serine deficiency in individuals with Neu-Laxova syndrome. This disorder thus seems to be an extremely severe expression of PHGDH deficiency.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PHGDH elisa kitPathways associated with PHGDH elisa kit
 Reference Product  PubMed Publications
 PSAT1 elisa kit  >8 publications with PHGDH and PSAT1
 PSPH elisa kit  >7 publications with PHGDH and PSPH
 ASNS elisa kit  >1 publications with PHGDH and ASNS
 Products by Pathway  Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
Diseases associated with PHGDH elisa kitOrgans/Tissues associated with PHGDH elisa kit
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >10 publications with PHGDH and Breast Neoplasms
 Melanoma Antibodies  >5 publications with PHGDH and Melanoma
 Nervous System Diseases Antibodies  >5 publications with PHGDH and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >5 publications with PHGDH and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >4 publications with PHGDH and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with PHGDH and Liver Diseases
 Phosphoglycerate Dehydrogenase Deficiency Antibodies  >3 publications with PHGDH and Phosphoglycerate Dehydrogenase Deficiency
 Brain Diseases Antibodies  >3 publications with PHGDH and Brain Diseases
 Neoplasms, Experimental Antibodies  >2 publications with PHGDH and Neoplasms, Experimental
 Memory Disorders Antibodies  >1 publications with PHGDH and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with PHGDH and Brain
 Liver Antibodies  >6 publications with PHGDH and Liver
 Blood Antibodies  >3 publications with PHGDH and Blood
 Nerve Antibodies  >2 publications with PHGDH and Nerve
 Embryonic Tissue Antibodies  >2 publications with PHGDH and Embryonic Tissue
 Bone Antibodies  >1 publications with PHGDH and Bone
 Muscle Antibodies  >1 publications with PHGDH and Muscle
 Uterus Antibodies  >1 publications with PHGDH and Uterus
 Kidney Antibodies  >1 publications with PHGDH and Kidney
 Lung Antibodies  >1 publications with PHGDH and Lung
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