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DNMT3A elisa kit :: Human DNMT3A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515871
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DNMT3A, ELISA Kit

★Popular Item★
 Also Known As   

Human DNMT3A(DNA Methyltransferase 3A)ELISA Kit

 Product Gene Name   

DNMT3A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602769
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanDNMT3A. No significant cross-reactivity or interference between HumanDNMT3A and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DNMT3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DNMT3A purchase    MBS2515871 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNMT3A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing DNMT3A, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2515871 kit is based on DNMT3A antibody-DNMT3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNMT3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNMT3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DNMT3A elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanDNMT3Aconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DNMT3A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DNMT3Aand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DNMT3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of DNMT3A.You can calculate the concentration of DNMT3Ain the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of DNMT3A elisa kit    DNMT3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515871. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DNMT3A. It may not necessarily be applicable to this product.
 NCBI GI #    77176455
 NCBI GeneID    1788
 NCBI Accession #    NP_715640.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_153759.3 [Other Products]
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 UniProt Secondary Accession #    Q86TE8; Q86XF5; Q8IZV0; Q8WXU9; E9PEB8 [Other Products]
 UniProt Related Accession #    Q9Y6K1 [Other Products]
 Molecular Weight    16,866 Da
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 NCBI Official Full Name    DNA (cytosine-5)-methyltransferase 3A isoform b
 NCBI Official Synonym Full Names    DNA (cytosine-5-)-methyltransferase 3 alpha
 NCBI Official Symbol    DNMT3A [Similar Products]
 NCBI Official Synonym Symbols   
TBRS; DNMT3A2; M.HsaIIIA
[Similar Products]
 NCBI Protein Information    DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2
 UniProt Protein Name    DNA (cytosine-5)-methyltransferase 3A
 UniProt Synonym Protein Names   
DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA
 Protein Family    DNA
 UniProt Gene Name    DNMT3A [Similar Products]
 UniProt Synonym Gene Names    Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA [Similar Products]
 UniProt Entry Name    DNM3A_HUMAN
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 NCBI Summary for DNMT3A    CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DNMT3A    DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase; EC 2.1.1.37; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; XY body; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; identical protein binding; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding; DNA-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; genetic imprinting; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; gene expression; DNA methylation during embryonic development; DNA methylation on cytosine

Disease: Tatton-brown-rahman Syndrome
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 Research Articles on DNMT3A    1. genetic variations in the DNMT3A1 promoter contribute to the susceptibility to gastric cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DNMT3A elisa kitPathways associated with DNMT3A elisa kit
 Reference Product  PubMed Publications
 DNMT3B elisa kit  >525 publications with DNMT3A and DNMT3B
 DNMT1 elisa kit  >519 publications with DNMT3A and DNMT1
 DNMT3L elisa kit  >118 publications with DNMT3A and DNMT3L
 EZH2 elisa kit  >60 publications with DNMT3A and EZH2
 MYC elisa kit  >19 publications with DNMT3A and MYC
 SUV39H1 elisa kit  >14 publications with DNMT3A and SUV39H1
 UHRF1 elisa kit  >12 publications with DNMT3A and UHRF1
 SETDB1 elisa kit  >7 publications with DNMT3A and SETDB1
 CBX5 elisa kit  >1 publications with DNMT3A and CBX5
 ZBTB17 elisa kit  >1 publications with DNMT3A and ZBTB17
 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 DNA Methylation Pathway antibodies  DNA Methylation Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
Diseases associated with DNMT3A elisa kitOrgans/Tissues associated with DNMT3A elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >36 publications with DNMT3A and Disease Models, Animal
 Adenocarcinoma Antibodies  >34 publications with DNMT3A and Adenocarcinoma
 Breast Neoplasms Antibodies  >30 publications with DNMT3A and Breast Neoplasms
 Liver Diseases Antibodies  >28 publications with DNMT3A and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >24 publications with DNMT3A and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >21 publications with DNMT3A and Lung Neoplasms
 Liver Neoplasms Antibodies  >21 publications with DNMT3A and Liver Neoplasms
 Ovarian Neoplasms Antibodies  >16 publications with DNMT3A and Ovarian Neoplasms
 Cardiovascular Diseases Antibodies  >15 publications with DNMT3A and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >15 publications with DNMT3A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >147 publications with DNMT3A and Blood
 Brain Antibodies  >88 publications with DNMT3A and Brain
 Liver Antibodies  >63 publications with DNMT3A and Liver
 Lung Antibodies  >38 publications with DNMT3A and Lung
 Embryonic Tissue Antibodies  >38 publications with DNMT3A and Embryonic Tissue
 Testis Antibodies  >27 publications with DNMT3A and Testis
 Prostate Antibodies  >25 publications with DNMT3A and Prostate
 Nerve Antibodies  >23 publications with DNMT3A and Nerve
 Muscle Antibodies  >19 publications with DNMT3A and Muscle
 Skin Antibodies  >19 publications with DNMT3A and Skin
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