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DPYD elisa kit :: Human DPYD ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512544
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DPYD, ELISA Kit

★Popular Item★
 Also Known As   

Human DPYD (Dihydropyrimidine Dehydrogenase) ELISA Kit

 Product Gene Name   

DPYD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    274270
 3D Structure    ModBase 3D Structure for Q12882
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human DPYD. No significant cross-reactivity or interference between Human DPYD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human DPYD and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156--10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DPYD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DPYD purchase    MBS2512544 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DPYD, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156--10ng/mL in biological research samples containing DPYD, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2512544 kit is based on DPYD antibody-DPYD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPYD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPYD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DPYD elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPYD in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DPYD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human DPYD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DPYD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DPYD. You can calculate the concentration of Human DPYD in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of DPYD elisa kit    DPYD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512544. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DPYD. It may not necessarily be applicable to this product.
 NCBI GI #    237757300
 NCBI GeneID    1806
 NCBI Accession #    NP_001153773.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001160301.1 [Other Products]
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 UniProt Primary Accession #    Q12882 [Other Products]
 UniProt Secondary Accession #    Q16694; Q16761; Q32NB0; Q96HL6; Q96TH1; A2RRQ2; A2RRQ3; A8K5A2; A8MWG9; B1AN21; E9PFN1 [Other Products]
 UniProt Related Accession #    Q12882 [Other Products]
 Molecular Weight    18,936 Da
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 NCBI Official Full Name    dihydropyrimidine dehydrogenase
 NCBI Official Synonym Full Names    dihydropyrimidine dehydrogenase
 NCBI Official Symbol    DPYD [Similar Products]
 NCBI Official Synonym Symbols   
DHP; DPD; DHPDHASE
[Similar Products]
 NCBI Protein Information    dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase
 UniProt Protein Name    Dihydropyrimidine dehydrogenase [NADP(+)]
 UniProt Synonym Protein Names   
Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase
 Protein Family    Dihydropyrimidine dehydrogenase
 UniProt Gene Name    DPYD [Similar Products]
 UniProt Synonym Gene Names    DHPDHase; DPD [Similar Products]
 UniProt Entry Name    DPYD_HUMAN
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 NCBI Summary for DPYD    The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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 UniProt Comments for DPYD    DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Oxidoreductase; Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; 4 iron, 4 sulfur cluster binding; dihydroorotate dehydrogenase activity; metal ion binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; UMP biosynthetic process; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; pyrimidine base catabolic process; thymidine catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency
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 Research Articles on DPYD    1. Pre-therapeutic DPYD testing may prevent 20-30% of life-threatening or lethal episodes of fluoropyrimidine toxicity in Caucasian patients
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DPYD elisa kitPathways associated with DPYD elisa kit
 Reference Product  PubMed Publications
 TYMP elisa kit  >8 publications with DPYD and TYMP
 CYP2A6 elisa kit  >3 publications with DPYD and CYP2A6
 UPB1 elisa kit  >2 publications with DPYD and UPB1
 DPYS elisa kit  >2 publications with DPYD and DPYS
 UPP1 elisa kit  >1 publications with DPYD and UPP1
 UMPS elisa kit  >1 publications with DPYD and UMPS
 Products by Pathway  Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
 Pantothenate And CoA Biosynthesis Pathway antibodies  Pantothenate And CoA Biosynthesis Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram
 Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway antibodies  Pyrimidine Degradation, Uracil => Beta-alanine, Thymine => 3-aminoisobutanoate Pathway Diagram
Diseases associated with DPYD elisa kitOrgans/Tissues associated with DPYD elisa kit
 Disease Name  Pubmed Publications
 Dihydropyrimidine Dehydrogenase Deficiency Antibodies  >74 publications with DPYD and Dihydropyrimidine Dehydrogenase Deficiency
 Gastrointestinal Diseases Antibodies  >66 publications with DPYD and Gastrointestinal Diseases
 Gastrointestinal Neoplasms Antibodies  >64 publications with DPYD and Gastrointestinal Neoplasms
 Colorectal Neoplasms Antibodies  >55 publications with DPYD and Colorectal Neoplasms
 Carcinoma Antibodies  >36 publications with DPYD and Carcinoma
 Purine-Pyrimidine Metabolism, Inborn Errors Antibodies  >36 publications with DPYD and Purine-Pyrimidine Metabolism, Inborn Errors
 Breast Neoplasms Antibodies  >21 publications with DPYD and Breast Neoplasms
 Adenocarcinoma Antibodies  >19 publications with DPYD and Adenocarcinoma
 Diarrhea Antibodies  >19 publications with DPYD and Diarrhea
 Neutropenia Antibodies  >17 publications with DPYD and Neutropenia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >65 publications with DPYD and Blood
 Bone Antibodies  >30 publications with DPYD and Bone
 Liver Antibodies  >16 publications with DPYD and Liver
 Connective Tissue Antibodies  >10 publications with DPYD and Connective Tissue
 Lung Antibodies  >8 publications with DPYD and Lung
 Prostate Antibodies  >6 publications with DPYD and Prostate
 Skin Antibodies  >5 publications with DPYD and Skin
 Brain Antibodies  >5 publications with DPYD and Brain
 Kidney Antibodies  >4 publications with DPYD and Kidney
 Nerve Antibodies  >4 publications with DPYD and Nerve
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