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| Product Name
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Defensin Alpha 5, Paneth Cell Specific (DEFa5), ELISA Kit ★Popular Item★
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| Also Known As
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Human Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit
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| Product Synonym Names
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DEF-A5; DEF5; HD-5
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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M97925 Genomic DNA
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| 3D Structure
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ModBase 3D Structure for Q01523
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of DEFa5. No significant cross-reactivity or interference between DEFa5 and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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| Assay Type
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Sandwich
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| Detection Range
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3.125-200pg/mL
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| Sensitivity
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1.31pg/mL
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFa5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
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| Inter-assay Precision
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Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFa5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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| Preparation and Storage
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For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of DEFa5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for DEFa5 purchase
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MBS455303 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Alpha 5, Paneth Cell Specific (DEFa5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.125-200pg/mL in biological research samples containing DEFa5, with an estimated sensitivity of 1.31pg/mL. The ELISA analytical biochemical technique of the MBS455303 kit is based on DEFa5 antibody-DEFa5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFa5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFa5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFa5 elisa kit
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Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DEFa5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFa5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DEFa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DEFa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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| Typical Testing Data/Standard Curve (for reference only) of DEFa5 elisa kit
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| Sample Manual Insert of MBS455303. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for DEFa5. It may not necessarily be applicable to this product. |
| NCBI GI #
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10337585
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| NCBI GeneID
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1670
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| NCBI Accession #
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NP_066290.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_021010.2
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[Other Products]
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| UniProt Primary Accession #
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Q01523
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[Other Products]
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| UniProt Secondary Accession #
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Q3KNV2; A0JDY6
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[Other Products]
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| UniProt Related Accession #
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Q01523
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[Other Products]
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| Molecular Weight
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10,071 Da
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| NCBI Official Full Name
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defensin-5 preproprotein
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| NCBI Official Synonym Full Names
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defensin alpha 5
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| NCBI Official Symbol
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DEFA5
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[Similar Products]
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| NCBI Official Synonym Symbols
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DEF5; HD-5
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[Similar Products]
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| NCBI Protein Information
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defensin-5
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| UniProt Protein Name
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Defensin-5
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| UniProt Synonym Protein Names
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Defensin, alpha 5; HD5(20-94)
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| Protein Family
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Defensin
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| UniProt Gene Name
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DEFA5
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[Similar Products]
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| UniProt Synonym Gene Names
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DEF5
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[Similar Products]
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| NCBI Summary for DEFa5
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Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]
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| UniProt Comments for DEFa5
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Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
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| Research Articles on DEFa5
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1. a moderately active linear peptide derived from the alpha-defensin HD5 can be engineered to enhance antimicrobial activity almost comparable to the native peptide.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with DEFa5 elisa kit | Pathways associated with DEFa5 elisa kit |
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| Diseases associated with DEFa5 elisa kit | Organs/Tissues associated with DEFa5 elisa kit |
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ELISA Kit 
