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DEFB103B elisa kit :: Human Defensin Beta 103B (DEFB103B) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020601 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
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10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Defensin Beta 103B (DEFB103B), ELISA Kit

★Popular Item★
 Also Known As   

Defensin Beta 103B (DEFB103B) ELISA Kit

 Product Synonym Names    HBP3; HBD3; BD3; DEFB103; DEFB3; Defensin-like protein; Beta-defensin 3
 Product Gene Name   

DEFB103B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606611
 3D Structure    ModBase 3D Structure for P81534
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Defensin Beta 103B (DEFB103B).
No significant cross-reactivity or interference between Defensin Beta 103B (DEFB103B) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    31.25-2000pg/mL
 Sensitivity    < 12.7pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFB103B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFB103B purchase    MBS2020601 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 103B (DEFB103B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing DEFB103B, with an estimated sensitivity of < 12.7pg/mL. The ELISA analytical biochemical technique of the MBS2020601 kit is based on DEFB103B antibody-DEFB103B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFB103B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFB103B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFB103B elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for DEFB103B elisa kit    Immune molecule
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 Typical Testing Data/Standard Curve (for reference only) of DEFB103B elisa kit    DEFB103B elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020601. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFB103B. It may not necessarily be applicable to this product.
 NCBI GI #    151555097
 NCBI GeneID    414325
 NCBI Accession #    AAI48675.1 [Other Products]
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 UniProt Primary Accession #    P81534 [Other Products]
 UniProt Secondary Accession #    Q8NFG6; Q9NPF6 [Other Products]
 UniProt Related Accession #    P81534 [Other Products]
 Molecular Weight    7,697 Da
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 NCBI Official Full Name    Defensin, beta 103B, partial
 NCBI Official Synonym Full Names    defensin, beta 103A
 NCBI Official Symbol    DEFB103A [Similar Products]
 NCBI Official Synonym Symbols   
BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103
[Similar Products]
 NCBI Protein Information    beta-defensin 103
 UniProt Protein Name    Beta-defensin 103
 UniProt Synonym Protein Names   
Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein
 UniProt Gene Name    DEFB103A [Similar Products]
 UniProt Synonym Gene Names    BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3 [Similar Products]
 UniProt Entry Name    D103A_HUMAN
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 NCBI Summary for DEFB103B    Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]
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 UniProt Comments for DEFB103B    defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
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 Research Articles on DEFB103B    1. The encoded peptide displays antimicrobial activity against S. aureus and E. faecium.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DEFB103B elisa kitPathways associated with DEFB103B elisa kit
 Reference Product  PubMed Publications
 CAMP elisa kit  >2 publications with DEFB103B and CAMP
 DEFB104A elisa kit  >2 publications with DEFB103B and DEFB104A
 Products by Pathway  Pathway Diagram
 Beta Defensins Pathway antibodies  Beta Defensins Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Diseases associated with DEFB103B elisa kit
 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >1 publications with DEFB103B and Metabolic Diseases
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