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DEFb126 elisa kit :: Human Defensin Beta 126 (DEFb126) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021586 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
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 Product Name   

Defensin Beta 126 (DEFb126), ELISA Kit

 Also Known As   

Defensin Beta 126 (DEFb126) ELISA Kit

 Product Synonym Names    ESP13.2; C20orf8; DEFB-26; Epididymal secretory protein 13.2
 Product Gene Name   

DEFb126 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9BYW3
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Defensin Beta 126 (DEFb126).
No significant cross-reactivity or interference between Defensin Beta 126 (DEFb126) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    7.8-500pg/mL
 Sensitivity    < 3.0pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Defensin Beta 126 (DEFb126) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Defensin Beta 126 (DEFb126) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DEFb126 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DEFb126 purchase    MBS2021586 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin Beta 126 (DEFb126) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8-500pg/mL in biological research samples containing DEFb126, with an estimated sensitivity of < 3.0pg/mL. The ELISA analytical biochemical technique of the MBS2021586 kit is based on DEFb126 antibody-DEFb126 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFb126 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFb126. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for DEFb126 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for DEFb126 elisa kit    Immune molecule
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 Typical Testing Data/Standard Curve (for reference only) of DEFb126 elisa kit    DEFb126 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021586. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DEFb126. It may not necessarily be applicable to this product.
 NCBI GI #    22252927
 NCBI GeneID    81623
 NCBI Accession #    AAM93919.1 [Other Products]
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 UniProt Primary Accession #    Q9BYW3 [Other Products]
 UniProt Secondary Accession #    Q562G3; Q9H1M5 [Other Products]
 UniProt Related Accession #    Q9BYW3 [Other Products]
 Molecular Weight    12,174 Da
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 NCBI Official Full Name    defensin beta 126, partial
 NCBI Official Synonym Full Names    defensin, beta 126
 NCBI Official Symbol    DEFB126 [Similar Products]
 NCBI Official Synonym Symbols   
DEFB26; hBD-26; C20orf8; DEFB-26; bA530N10.1
[Similar Products]
 NCBI Protein Information    beta-defensin 126
 UniProt Protein Name    Beta-defensin 126
 UniProt Synonym Protein Names   
Beta-defensin 26; DEFB-26; Defensin, beta 126; Epididymal secretory protein 13.2; ESP13.2
 Protein Family    Beta-defensin
 UniProt Gene Name    DEFB126 [Similar Products]
 UniProt Synonym Gene Names    C20orf8; DEFB26; DEFB-26; ESP13.2 [Similar Products]
 UniProt Entry Name    DB126_HUMAN
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 NCBI Summary for DEFb126    Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. The encoded protein is highly similar to an epididymal-specific secretory protein (ESP13.2) from cynomolgus monkey. Mutation of this gene is associated with impaired sperm function. [provided by RefSeq, Nov 2014]
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 UniProt Comments for DEFb126    defensin, beta 26: Has antibacterial activity (Potential). Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: glycocalyx; cell surface; extracellular region

Biological Process: defense response to bacterium; innate immune response
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 Research Articles on DEFb126    1. These results demonstrate that the DEFB126 core peptide has critical functions in parallel to its C-terminal tail by showing LPS-binding activity, anti-inflammatory effects and intracellular regulatory function. In addition, DEFB126 displays antimicrobial activity against E. coli, S. aureus, and C. elegans.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DEFb126 elisa kitPathways associated with DEFb126 elisa kit
 Reference Product  PubMed Publications
 DEFB106A elisa kit  >1 publications with DEFb126 and DEFB106A
 SPINLW1 elisa kit  >1 publications with DEFb126 and SPINLW1
 DEFB129 elisa kit  >1 publications with DEFb126 and DEFB129
 Products by Pathway  Pathway Diagram
 Beta Defensins Pathway antibodies  Beta Defensins Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
Organs/Tissues associated with DEFb126 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >36 publications with DEFb126 and Epididymis
 Testis Antibodies  >3 publications with DEFb126 and Testis
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