Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow DAO rightarrow Human DAO  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • VISA (MAVS) Antibody
 • TSH Receptor (TSHR) Antibody
 • DNA, Salmon Testes Denatured, 5mg-ml DNA
 • ER Antibody
 • T7 (THVgp007) Antibody
 • guanine nucleotide binding protein (G protein), gamma transd... Antibody
 • Galectin 9 (GAL9) ELISA Kit
 • GAA Antibody
 • Glutathione (GT) ELISA Kit
 • c-Jun Antibody
 • Transferrin Receptor (TFRC) Antibody
 • leucine aminopeptidase 3 (LAP3) ELISA Kit
 • Ki67 Antibody
 • JAW1 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

DAO elisa kit :: Human Diamine Oxidase (DAO) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS450322
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $390 +1 FREE 8GB USB
96-Strip-Wells  /  $510 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,225 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,320 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Diamine Oxidase (DAO), ELISA Kit

 Also Known As   

Human Diamine Oxidase (DAO) ELISA Kit

 Product Synonym Names    AKP; ALKP; Basic Phosphatase
 Product Gene Name   

DAO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    M55602 mRNA
 3D Structure    ModBase 3D Structure for P19801
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of DAO. No significant cross-reactivity or interference between DAO and analogues was observed.
Section Bar
 Samples    Human serum, plasma, tissue homogenates, and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.312-20ng/mL
 Sensitivity    <0.127ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DAO were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DAO were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Section Bar
 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of DAO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DAO purchase    MBS450322 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Diamine Oxidase (DAO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing DAO, with an estimated sensitivity of <0.127ng/mL. The ELISA analytical biochemical technique of the MBS450322 kit is based on DAO antibody-DAO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DAO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DAO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma, tissue homogenates, and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for DAO elisa kit

   Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DAO in human serum, plasma, tissue homogenates, and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of DAO elisa kit    DAO elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS450322
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for DAO. It may not necessarily be applicable to this product.
 NCBI GI #    73486661
 NCBI GeneID    26
 NCBI Accession #    NP_001082.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001091.3 [Other Products]
Section Bar
 UniProt Primary Accession #    P19801 [Other Products]
 UniProt Secondary Accession #    Q16683; Q16684; Q56II4; Q6GU42; C9J690 [Other Products]
 UniProt Related Accession #    P19801 [Other Products]
 Molecular Weight    87,239 Da
Table BarTOPTable Bar
 NCBI Official Full Name    amiloride-sensitive amine oxidase
 NCBI Official Synonym Full Names    amine oxidase, copper containing 1
 NCBI Official Symbol    AOC1 [Similar Products]
 NCBI Official Synonym Symbols   
ABP; DAO; KAO; ABP1; DAO1
[Similar Products]
 NCBI Protein Information    amiloride-sensitive amine oxidase [copper-containing]
 UniProt Protein Name    Amiloride-sensitive amine oxidase [copper-containing]
 UniProt Synonym Protein Names   
Amiloride-binding protein 1; Amine oxidase copper domain-containing protein 1; Histaminase; Kidney amine oxidase; KAO
 Protein Family    D-amino-acid oxidase
 UniProt Gene Name    AOC1 [Similar Products]
 UniProt Synonym Gene Names    ABP1; DAO1; DAO; Diamine oxidase; KAO [Similar Products]
Section Bar
 NCBI Summary for DAO    This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Section Bar
 UniProt Comments for DAO    Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.
Table BarTOPTable Bar
 Research Articles on DAO    1. HRH1-17 TT and HNMT-1639 TT genotypes were associated with the allergic asthma phenotype among African-American children and that the ABP 4107 GG genotype was associated with nonallergic asthma among white children.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with DAO elisa kitDiseases associated with DAO elisa kit
 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Histidine Metabolism Pathway antibodies  Histidine Metabolism Pathway Diagram
 Histidine Metabolism Pathway antibodies  Histidine Metabolism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with DAO and Kidney Diseases
Organs/Tissues associated with DAO elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >4 publications with DAO and Kidney
 Blood Antibodies  >1 publications with DAO and Blood
 Liver Antibodies  >1 publications with DAO and Liver
 Intestine Antibodies  >1 publications with DAO and Intestine
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions