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DLD elisa kit :: Human Dihydrolipoyl dehydrogenase, mitochondrial ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2885103
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $430 +1 FREE 8GB USB
96-Strip-Wells  /  $570 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,515 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Dihydrolipoyl dehydrogenase, mitochondrial (DLD), ELISA Kit

★Popular Item★
 Also Known As   

Human Dihydrolipoyl dehydrogenase, mitochondrial ELISA Kit

 Product Synonym Names    Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; DLD; GCSL; LAD; PHE3; 1.8.1.4
 Product Gene Name   

DLD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2885103 Testing Data    Testing Data PDF
 OMIM    238331
 3D Structure    ModBase 3D Structure for P09622
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 Species Reactivity    Human
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 Assay Type    Competitive
 Detection Range    0.78-50 ng/mL
 Sensitivity    0.45 ng/mL
 Intra-Assay CV    <=8.4%
 Inter-Assay CV    <=11.1%
 Recovery    91%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DLD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DLD purchase    MBS2885103 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dihydrolipoyl dehydrogenase, mitochondrial (DLD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50 ng/mL in biological research samples containing DLD, with an estimated sensitivity of 0.45 ng/mL. The ELISA analytical biochemical technique of the MBS2885103 kit is based on DLD antibody-DLD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DLD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DLD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for DLD elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of DLD elisa kit    DLD elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2885103. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DLD. It may not necessarily be applicable to this product.
 NCBI GI #    91199540
 NCBI GeneID    1738
 NCBI Accession #    NP_000099.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000108.4 [Other Products]
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 UniProt Primary Accession #    P09622 [Other Products]
 UniProt Secondary Accession #    Q14131; Q14167; Q59EV8; Q8WTS4; B2R5X0; B4DHG0; B4DT69 [Other Products]
 UniProt Related Accession #    P09622 [Other Products]
 Molecular Weight    49,283 Da
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 NCBI Official Full Name    dihydrolipoyl dehydrogenase, mitochondrial isoform 1
 NCBI Official Synonym Full Names    dihydrolipoamide dehydrogenase
 NCBI Official Symbol    DLD [Similar Products]
 NCBI Official Synonym Symbols   
E3; LAD; DLDD; DLDH; GCSL; PHE3
[Similar Products]
 NCBI Protein Information    dihydrolipoyl dehydrogenase, mitochondrial
 UniProt Protein Name    Dihydrolipoyl dehydrogenase, mitochondrial
 UniProt Synonym Protein Names   
Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein
 Protein Family    Delta-like protein
 UniProt Gene Name    DLD [Similar Products]
 UniProt Synonym Gene Names    GCSL; LAD; PHE3 [Similar Products]
 UniProt Entry Name    DLDH_HUMAN
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 NCBI Summary for DLD    This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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 UniProt Comments for DLD    DLD: a multi-functional mitochondrial enzyme. An enzymatic component of the mitochondrial glycine cleavage system, the pyruvate dehydrogenase complex (PDHC), the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Is the E3 component of the PDHC that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. The E3 component has dihydrolipoamide dehydrogenase activity. The PDHC contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. Defects in DLD are a cause of maple syrup urine disease (MSUD), characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. Differentially expressed in the Wernicke's Area from patients with schizophrenia. Inhibited by 5-methoxyindole-2-carboxylic acid (MICA).

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.8.1.4

Chromosomal Location of Human Ortholog: 7q31-q32

Cellular Component: acrosomal matrix; cilium; mitochondrial matrix; mitochondrion; myelin sheath; nucleoplasm; oxoglutarate dehydrogenase complex; pyruvate dehydrogenase complex

Molecular Function: dihydrolipoyl dehydrogenase activity; FAD binding; lipoamide binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; aging; branched chain family amino acid catabolic process; cell redox homeostasis; cellular metabolic process; dihydrolipoamide metabolic process; gastrulation; glyoxylate metabolic process; lipoate metabolic process; lysine catabolic process; mitochondrial electron transport, NADH to ubiquinone; proteolysis; pyruvate metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of membrane potential; sperm capacitation; tricarboxylic acid cycle

Disease: Dihydrolipoamide Dehydrogenase Deficiency
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 Research Articles on DLD    1. Mitochondrial dihydrolipoamide dehydrogenase is upregulated in response to the brain intermittent hypoxic preconditioning.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DLD elisa kitPathways associated with DLD elisa kit
 Reference Product  PubMed Publications
 BCKDHA elisa kit  >7 publications with DLD and BCKDHA
 BCKDHB elisa kit  >6 publications with DLD and BCKDHB
 DBT elisa kit  >6 publications with DLD and DBT
 PDHA1 elisa kit  >4 publications with DLD and PDHA1
 PDHB elisa kit  >3 publications with DLD and PDHB
 DLAT elisa kit  >2 publications with DLD and DLAT
 DLST elisa kit  >2 publications with DLD and DLST
 PDHX elisa kit  >2 publications with DLD and PDHX
 Products by Pathway  Pathway Diagram
 2-oxobutanoate Degradation Pathway antibodies  2-oxobutanoate Degradation Pathway Diagram
 2-oxobutanoate Degradation I Pathway antibodies  2-oxobutanoate Degradation I Pathway Diagram
 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway antibodies  2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway Diagram
 2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway antibodies  2-oxoglutarate Decarboxylation To Succinyl-CoA Pathway Diagram
 2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway antibodies  2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway Diagram
 2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway antibodies  2-oxoisovalerate Decarboxylation To Isobutanoyl-CoA Pathway Diagram
 Branched-chain Amino Acid Catabolism Pathway antibodies  Branched-chain Amino Acid Catabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
Diseases associated with DLD elisa kitOrgans/Tissues associated with DLD elisa kit
 Disease Name  Pubmed Publications
 Death Antibodies  >130 publications with DLD and Death
 Nervous System Diseases Antibodies  >95 publications with DLD and Nervous System Diseases
 Lung Diseases Antibodies  >92 publications with DLD and Lung Diseases
 Necrosis Antibodies  >78 publications with DLD and Necrosis
 Neurobehavioral Manifestations Antibodies  >75 publications with DLD and Neurobehavioral Manifestations
 Neoplasms, Experimental Antibodies  >71 publications with DLD and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >58 publications with DLD and Liver Neoplasms
 Breast Neoplasms Antibodies  >43 publications with DLD and Breast Neoplasms
 Inflammation Antibodies  >32 publications with DLD and Inflammation
 Cell Transformation, Neoplastic Antibodies  >27 publications with DLD and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >152 publications with DLD and Lung
 Blood Antibodies  >143 publications with DLD and Blood
 Liver Antibodies  >81 publications with DLD and Liver
 Intestine Antibodies  >75 publications with DLD and Intestine
 Vascular Antibodies  >61 publications with DLD and Vascular
 Brain Antibodies  >53 publications with DLD and Brain
 Muscle Antibodies  >32 publications with DLD and Muscle
 Stomach Antibodies  >26 publications with DLD and Stomach
 Skin Antibodies  >25 publications with DLD and Skin
 Lymph Node Antibodies  >24 publications with DLD and Lymph Node
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