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ECM1 elisa kit :: Human ECM1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2505556
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ECM1, ELISA Kit

★Popular Item★
 Also Known As   

Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit

 Product Gene Name   

ECM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2505556 COA    COA PDF
 OMIM    247100
 3D Structure    ModBase 3D Structure for Q16610
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinant Human ECM1. No significant cross-reactivity or interference between Human ECM1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich
 Detection Range    31.25-2000pg/mL
 Sensitivity    18.75pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ECM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ECM1 purchase    MBS2505556 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ECM1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing ECM1, with an estimated sensitivity of 18.75pg/mL. The ELISA analytical biochemical technique of the MBS2505556 kit is based on ECM1 antibody-ECM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ECM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ECM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ECM1 elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ECM1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ECM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ECM1. You can calculate the concentration of ECM1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ECM1 elisa kit    ECM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2505556. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ECM1. It may not necessarily be applicable to this product.
 NCBI GI #    322302700
 NCBI GeneID    1893
 NCBI Accession #    NP_001189787.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001202858.1 [Other Products]
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 UniProt Primary Accession #    Q16610 [Other Products]
 UniProt Secondary Accession #    O43266; Q5T5G4; Q5T5G5; Q5T5G6; Q8IZ60; A8K8S0; B4DW49; B4DY60 [Other Products]
 UniProt Related Accession #    Q16610 [Other Products]
 Molecular Weight    63,563 Da
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 NCBI Official Full Name    extracellular matrix protein 1 isoform 3
 NCBI Official Synonym Full Names    extracellular matrix protein 1
 NCBI Official Symbol    ECM1 [Similar Products]
 NCBI Official Synonym Symbols   
URBWD
[Similar Products]
 NCBI Protein Information    extracellular matrix protein 1; secretory component p85
 UniProt Protein Name    Extracellular matrix protein 1
 UniProt Synonym Protein Names   
Secretory component p85
 Protein Family    Extracellular matrix protein
 UniProt Gene Name    ECM1 [Similar Products]
 UniProt Entry Name    ECM1_HUMAN
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 NCBI Summary for ECM1    This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
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 UniProt Comments for ECM1    ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space

Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; positive regulation of endothelial cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; regulation of T-helper 2 type immune response; inflammatory response

Disease: Lipoid Proteinosis Of Urbach And Wiethe
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 Research Articles on ECM1    1. The data supports the conclusion that the c.742G>T mutation nonsense mutation in ECM1 is the pathological cause of lipoid proteinosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with ECM1 elisa kitOrgans/Tissues associated with ECM1 elisa kit
 Disease Name  Pubmed Publications
 Lipoid Proteinosis of Urbach and Wiethe Antibodies  >51 publications with ECM1 and Lipoid Proteinosis of Urbach and Wiethe
 Carcinoma Antibodies  >21 publications with ECM1 and Carcinoma
 Breast Neoplasms Antibodies  >10 publications with ECM1 and Breast Neoplasms
 Inflammation Antibodies  >9 publications with ECM1 and Inflammation
 Colitis, Ulcerative Antibodies  >9 publications with ECM1 and Colitis, Ulcerative
 Carcinoma, Squamous Cell Antibodies  >6 publications with ECM1 and Carcinoma, Squamous Cell
 Adenocarcinoma Antibodies  >5 publications with ECM1 and Adenocarcinoma
 Disease Models, Animal Antibodies  >3 publications with ECM1 and Disease Models, Animal
 Precancerous Conditions Antibodies  >2 publications with ECM1 and Precancerous Conditions
 Neurobehavioral Manifestations Antibodies  >2 publications with ECM1 and Neurobehavioral Manifestations
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >57 publications with ECM1 and Skin
 Blood Antibodies  >26 publications with ECM1 and Blood
 Vascular Antibodies  >19 publications with ECM1 and Vascular
 Bone Antibodies  >10 publications with ECM1 and Bone
 Brain Antibodies  >9 publications with ECM1 and Brain
 Lymph Node Antibodies  >7 publications with ECM1 and Lymph Node
 Muscle Antibodies  >6 publications with ECM1 and Muscle
 Thyroid Antibodies  >5 publications with ECM1 and Thyroid
 Connective Tissue Antibodies  >5 publications with ECM1 and Connective Tissue
 Mouth Antibodies  >5 publications with ECM1 and Mouth
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