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EGFL7 elisa kit :: Human EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024627 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EGF Like Domain Protein, Multiple 7 (EGFL7), ELISA Kit

★Popular Item★
 Also Known As   

EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit

 Product Synonym Names    ZNEU1; MEGF7; Zneu1; Vascular endothelial statin; EGF Like Domain Containing 7; Epidermal growth factor-like protein 7; Multiple epidermal growth factor-like domains protein 7
 Product Gene Name   

EGFL7 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608582
 3D Structure    ModBase 3D Structure for Q9UHF1
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of EGF Like Domain Protein, Multiple 7 (EGFL7).
No significant cross-reactivity or interference between EGF Like Domain Protein, Multiple 7 (EGFL7) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    31.25-2000pg/mL
 Sensitivity    < 13.1pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level EGF Like Domain Protein, Multiple 7 (EGFL7) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EGFL7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EGFL7 purchase    MBS2024627 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25-2000pg/mL in biological research samples containing EGFL7, with an estimated sensitivity of < 13.1pg/mL. The ELISA analytical biochemical technique of the MBS2024627 kit is based on EGFL7 antibody-EGFL7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGFL7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGFL7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EGFL7 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 7 (EGFL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 7 (EGFL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGF Like Domain Protein, Multiple 7 (EGFL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for EGFL7 elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of EGFL7 elisa kit    EGFL7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024627. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EGFL7. It may not necessarily be applicable to this product.
 NCBI GI #    41393583
 NCBI GeneID    51162
 NCBI Accession #    NP_958854.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_201446.2 [Other Products]
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 UniProt Primary Accession #    Q9UHF1 [Other Products]
 UniProt Secondary Accession #    Q5M7Y5; Q5VUD5; Q96EG0; B3KRP0; M9VTX9 [Other Products]
 UniProt Related Accession #    Q9UHF1 [Other Products]
 Molecular Weight    29,618 Da
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 NCBI Official Full Name    epidermal growth factor-like protein 7
 NCBI Official Synonym Full Names    EGF-like-domain, multiple 7
 NCBI Official Symbol    EGFL7 [Similar Products]
 NCBI Official Synonym Symbols   
NEU1; ZNEU1; VE-STATIN
[Similar Products]
 NCBI Protein Information    epidermal growth factor-like protein 7
 UniProt Protein Name    Epidermal growth factor-like protein 7
 UniProt Synonym Protein Names   
Multiple epidermal growth factor-like domains protein 7; Multiple EGF-like domains protein 7; NOTCH4-like protein; Vascular endothelial statin; VE-statin; Zneu1
 Protein Family    Epidermal growth factor-like protein
 UniProt Gene Name    EGFL7 [Similar Products]
 UniProt Synonym Gene Names    MEGF7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin [Similar Products]
 UniProt Entry Name    EGFL7_HUMAN
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 NCBI Summary for EGFL7    This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
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 UniProt Comments for EGFL7    EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: calcium ion binding; Notch binding

Biological Process: blood vessel development; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; angiogenesis; vasculogenesis; cell adhesion; negative regulation of cell migration
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 Research Articles on EGFL7    1. demonstrate significantly reduced Egfl7 expression in pre-eclampsia placentas, concurrent with a downregulation of Notch target genes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EGFL7 elisa kitDiseases associated with EGFL7 elisa kit
 Reference Product  PubMed Publications
 SPRED1 elisa kit  >4 publications with EGFL7 and SPRED1
 ICAM1 elisa kit  >4 publications with EGFL7 and ICAM1
 LOXL2 elisa kit  >1 publications with EGFL7 and LOXL2
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >22 publications with EGFL7 and Carcinoma
 Carcinoma, Hepatocellular Antibodies  >7 publications with EGFL7 and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >7 publications with EGFL7 and Lung Neoplasms
 Liver Diseases Antibodies  >6 publications with EGFL7 and Liver Diseases
 Death Antibodies  >5 publications with EGFL7 and Death
 Inflammation Antibodies  >5 publications with EGFL7 and Inflammation
 Liver Neoplasms Antibodies  >4 publications with EGFL7 and Liver Neoplasms
 Prostatic Diseases Antibodies  >2 publications with EGFL7 and Prostatic Diseases
 Congenital Abnormalities Antibodies  >2 publications with EGFL7 and Congenital Abnormalities
 Heart Diseases Antibodies  >2 publications with EGFL7 and Heart Diseases
Organs/Tissues associated with EGFL7 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >62 publications with EGFL7 and Vascular
 Blood Antibodies  >44 publications with EGFL7 and Blood
 Lung Antibodies  >13 publications with EGFL7 and Lung
 Embryonic Tissue Antibodies  >13 publications with EGFL7 and Embryonic Tissue
 Heart Antibodies  >8 publications with EGFL7 and Heart
 Liver Antibodies  >8 publications with EGFL7 and Liver
 Brain Antibodies  >7 publications with EGFL7 and Brain
 Muscle Antibodies  >5 publications with EGFL7 and Muscle
 Skin Antibodies  >3 publications with EGFL7 and Skin
 Ovary Antibodies  >3 publications with EGFL7 and Ovary
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