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EPHA4 elisa kit :: Human EPH receptor A4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS914925
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EPH receptor A4 (EPHA4), ELISA Kit

 Also Known As   

Human Ephrin type-A receptor 4, EPHA4 ELISA Kit

 Product Synonym Names    Human Ephrin type-A receptor 4 (EPHA4) ELISA kit; HEK8; SEK; TYRO1; OTTHUMP00000164185; TYRO1 protein tyrosine kinase; ephrin receptor EphA4; ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; tyrosine-protein kinase receptor SEK; EPH receptor A4
 Product Gene Name   

EPHA4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS914925 Testing Data    Testing Data PDF
 OMIM    602188
 3D Structure    ModBase 3D Structure for P54764
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human EPHA4. No significant cross-reactivity or interference between human EPHA4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    25 pg/ml -1600 pg/ml.
 Sensitivity    The minimum detectable dose of human EPHA4is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of EPHA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPHA4 purchase    MBS914925 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EPH receptor A4 (EPHA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25 pg/ml -1600 pg/ml in biological research samples containing EPHA4, with an estimated sensitivity of The minimum detectable dose of human EPHA4is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS914925 kit is based on EPHA4 antibody-EPHA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPHA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPHA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for EPHA4 elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of EPHA4 elisa kit    EPHA4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS914925
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NCBI/Uniprot data below describe general gene information for EPHA4. It may not necessarily be applicable to this product.
 NCBI GI #    4758280
 NCBI GeneID    2043
 NCBI Accession #    NP_004429.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004438.3 [Other Products]
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 UniProt Primary Accession #    P54764 [Other Products]
 UniProt Secondary Accession #    Q2M380; A8K2P1; B2R601 [Other Products]
 UniProt Related Accession #    P54764 [Other Products]
 Molecular Weight    109,860 Da
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 NCBI Official Full Name    ephrin type-A receptor 4
 NCBI Official Synonym Full Names    EPH receptor A4
 NCBI Official Symbol    EPHA4 [Similar Products]
 NCBI Official Synonym Symbols   
SEK; HEK8; TYRO1
[Similar Products]
 NCBI Protein Information    ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8
 UniProt Protein Name    Ephrin type-A receptor 4
 UniProt Synonym Protein Names   
EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK
 Protein Family    Ephrin type-A receptor
 UniProt Gene Name    EPHA4 [Similar Products]
 UniProt Synonym Gene Names    HEK8; SEK; TYRO1; EK8; hEK8 [Similar Products]
 UniProt Entry Name    EPHA4_HUMAN
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 NCBI Summary for EPHA4    This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EPHA4    EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; early endosome membrane; axon; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium

Molecular Function: identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; ephrin receptor signaling pathway; regulation of astrocyte differentiation; motor axon guidance; positive regulation of dendrite morphogenesis; cell adhesion; corticospinal tract morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior
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 Research Articles on EPHA4    1. Data indicate that ephrin-A5 binding directly facilitates the formation of Eph/ephrin clusters by inducing conformational changes in the ligand-binding domain (LBD) of EphA4.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EPHA4 elisa kitPathways associated with EPHA4 elisa kit
 Reference Product  PubMed Publications
 EFNB3 elisa kit  >23 publications with EPHA4 and EFNB3
 FGFR1 elisa kit  >5 publications with EPHA4 and FGFR1
 FYN elisa kit  >5 publications with EPHA4 and FYN
 NGEF elisa kit  >3 publications with EPHA4 and NGEF
 EFNB2 elisa kit  >2 publications with EPHA4 and EFNB2
 EFNA3 elisa kit  >2 publications with EPHA4 and EFNA3
 FGFR4 elisa kit  >1 publications with EPHA4 and FGFR4
 FGFR3 elisa kit  >1 publications with EPHA4 and FGFR3
 FGFR2 elisa kit  >1 publications with EPHA4 and FGFR2
 EFNA5 elisa kit  >1 publications with EPHA4 and EFNA5
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 EPHA Forward Signaling Pathway antibodies  EPHA Forward Signaling Pathway Diagram
 EphrinA-EPHA Pathway antibodies  EphrinA-EPHA Pathway Diagram
Diseases associated with EPHA4 elisa kitOrgans/Tissues associated with EPHA4 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >34 publications with EPHA4 and Nervous System Diseases
 Neoplasms Antibodies  >23 publications with EPHA4 and Neoplasms
 Brain Diseases Antibodies  >10 publications with EPHA4 and Brain Diseases
 Brain Injuries Antibodies  >8 publications with EPHA4 and Brain Injuries
 Cardiovascular Diseases Antibodies  >7 publications with EPHA4 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >6 publications with EPHA4 and Vascular Diseases
 Immune System Diseases Antibodies  >4 publications with EPHA4 and Immune System Diseases
 Inflammation Antibodies  >4 publications with EPHA4 and Inflammation
 Necrosis Antibodies  >4 publications with EPHA4 and Necrosis
 Breast Neoplasms Antibodies  >2 publications with EPHA4 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >151 publications with EPHA4 and Brain
 Embryonic Tissue Antibodies  >55 publications with EPHA4 and Embryonic Tissue
 Muscle Antibodies  >32 publications with EPHA4 and Muscle
 Eye Antibodies  >24 publications with EPHA4 and Eye
 Blood Antibodies  >23 publications with EPHA4 and Blood
 Bone Antibodies  >21 publications with EPHA4 and Bone
 Vascular Antibodies  >16 publications with EPHA4 and Vascular
 Skin Antibodies  >8 publications with EPHA4 and Skin
 Kidney Antibodies  >8 publications with EPHA4 and Kidney
 Connective Tissue Antibodies  >6 publications with EPHA4 and Connective Tissue
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