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ET-1 elisa kit :: Human ET-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502693 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $310 +1 FREE 8GB USB
96-Strip-Wells  /  $355 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ET-1, ELISA Kit

★Popular Item★
 Also Known As   

Human ET-1 (Endothelin 1) ELISA Kit

 Product Gene Name   

ET-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P13206
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ET-1. No significant cross-reactivity or interference between Human ET-1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ET-1 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    1.25--80pg/mL
 Sensitivity    0.75pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ET-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ET-1 purchase    MBS2502693 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ET-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25--80pg/mL in biological research samples containing ET-1, with an estimated sensitivity of 0.75pg/mL. The ELISA analytical biochemical technique of the MBS2502693 kit is based on ET-1 antibody-ET-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ET-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ET-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ET-1 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ET-1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ET-1 elisa kit    ET-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502693. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ET-1. It may not necessarily be applicable to this product.
 NCBI GI #    50950175
 NCBI GeneID    403424
 NCBI Accession #    NP_001002956.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001002956.1 [Other Products]
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 UniProt Primary Accession #    P13206 [Other Products]
 UniProt Secondary Accession #    Q765Z3; Q9BDC1 [Other Products]
 UniProt Related Accession #    P13206 [Other Products]
 Molecular Weight    22,977 Da
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 NCBI Official Full Name    endothelin-1
 NCBI Official Symbol    EDN1 [Similar Products]
 NCBI Official Synonym Symbols   
PPET1
[Similar Products]
 NCBI Protein Information    endothelin-1; ET-1; gene for endothelin; preproendothelin-1
 UniProt Protein Name    Endothelin-1
 UniProt Synonym Protein Names   
Preproendothelin-1; PPET1
 UniProt Gene Name    EDN1 [Similar Products]
 UniProt Synonym Gene Names    ET-1; PPET1 [Similar Products]
 UniProt Entry Name    EDN1_CANFA
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 UniProt Comments for ET-1    Endothelins are endothelium-derived vasoconstrictor peptides.
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 Research Articles on ET-1    1. These results showed that endothelin-1 and endothelin receptor A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ET-1 elisa kitDiseases associated with ET-1 elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >38 publications with ET-1 and Cardiovascular Diseases
 Hypertension Antibodies  >31 publications with ET-1 and Hypertension
 Inflammation Antibodies  >20 publications with ET-1 and Inflammation
 Craniofacial Abnormalities Antibodies  >19 publications with ET-1 and Craniofacial Abnormalities
 Asthma Antibodies  >13 publications with ET-1 and Asthma
 Kidney Diseases Antibodies  >8 publications with ET-1 and Kidney Diseases
 Cardiovascular Abnormalities Antibodies  >7 publications with ET-1 and Cardiovascular Abnormalities
 Hypertension, Pulmonary Antibodies  >6 publications with ET-1 and Hypertension, Pulmonary
 Heart Failure Antibodies  >5 publications with ET-1 and Heart Failure
 Diabetes Mellitus, Type 2 Antibodies  >5 publications with ET-1 and Diabetes Mellitus, Type 2
Organs/Tissues associated with ET-1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >23 publications with ET-1 and Muscle
 Brain Antibodies  >12 publications with ET-1 and Brain
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