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eNOS elisa kit :: Human Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265088
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Endothelial Nitric Oxide Synthase (eNOS), ELISA Kit

★Popular Item★
 Also Known As   

Human Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit

 Product Gene Name   

eNOS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    163729
 3D Structure    ModBase 3D Structure for P29474
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant eNOS concentration.
 Assay Type    Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    Up to 0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of eNOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for eNOS purchase    MBS265088 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endothelial Nitric Oxide Synthase (eNOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing eNOS, with an estimated sensitivity of Up to 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS265088 kit is based on eNOS antibody-eNOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect eNOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, eNOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant eNOS concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for eNOS elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human eNOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of eNOS elisa kit   
eNOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS265088. Click to request current manual
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NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.
 NCBI GI #    825652
 NCBI GeneID    4846
 NCBI Accession #    CAA53950.1 [Other Products]
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 UniProt Primary Accession #    P29474 [Other Products]
 UniProt Secondary Accession #    Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2 [Other Products]
 UniProt Related Accession #    P29474 [Other Products]
 Molecular Weight    67,876 Da
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 NCBI Official Full Name    endothelial nitric oxide synthase
 NCBI Official Synonym Full Names    nitric oxide synthase 3 (endothelial cell)
 NCBI Official Symbol    NOS3 [Similar Products]
 NCBI Official Synonym Symbols   
eNOS; ECNOS
[Similar Products]
 NCBI Protein Information    nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3 transcript variant eNOS-delta20-21
 UniProt Protein Name    Nitric oxide synthase, endothelial
 UniProt Synonym Protein Names   
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
 UniProt Gene Name    NOS3 [Similar Products]
 UniProt Synonym Gene Names    cNOS; eNOS; NOSIII [Similar Products]
 UniProt Entry Name    NOS3_HUMAN
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 NCBI Summary for eNOS    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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 UniProt Comments for eNOS    eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; FMN binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; regulation of blood pressure; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; angiogenesis; negative regulation of blood pressure; nitric oxide biosynthetic process; regulation of blood vessel size; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; smooth muscle hyperplasia; lung development

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
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 Research Articles on eNOS    1. study to determine the association between the eNOS 4a/b polymorphism and breast cancer in Mexican women; genotypes a/a-a/b are associated with breast cancer susceptibility in the analyzed samples from the Mexican population
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with eNOS elisa kitDiseases associated with eNOS elisa kit
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >1908 publications with eNOS and Cardiovascular Diseases
 Hypertension Antibodies  >943 publications with eNOS and Hypertension
 Myocardial Ischemia Antibodies  >514 publications with eNOS and Myocardial Ischemia
 Ischemia Antibodies  >492 publications with eNOS and Ischemia
 Atherosclerosis Antibodies  >423 publications with eNOS and Atherosclerosis
 Coronary Artery Disease Antibodies  >346 publications with eNOS and Coronary Artery Disease
 Arteriosclerosis Antibodies  >321 publications with eNOS and Arteriosclerosis
 Reperfusion Injury Antibodies  >247 publications with eNOS and Reperfusion Injury
 Myocardial Infarction Antibodies  >222 publications with eNOS and Myocardial Infarction
 Neoplasms Antibodies  >198 publications with eNOS and Neoplasms
Organs/Tissues associated with eNOS elisa kit
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3282 publications with eNOS and Vascular
 Blood Antibodies  >2461 publications with eNOS and Blood
 Heart Antibodies  >1134 publications with eNOS and Heart
 Kidney Antibodies  >479 publications with eNOS and Kidney
 Brain Antibodies  >405 publications with eNOS and Brain
 Lung Antibodies  >347 publications with eNOS and Lung
 Liver Antibodies  >242 publications with eNOS and Liver
 Embryonic Tissue Antibodies  >199 publications with eNOS and Embryonic Tissue
 Bone Antibodies  >129 publications with eNOS and Bone
 Skin Antibodies  >90 publications with eNOS and Skin
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