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EPAS1 elisa kit :: Human Endothelial PAS Domain-Containing Protein 1 (EPAS1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9361593 EPAS1 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Endothelial PAS Domain-Containing Protein 1 (EPAS1), ELISA Kit

★Popular Item★
 Also Known As   

Human Endothelial PAS Domain-Containing Protein 1 (EPAS1) ELISA Kit

 Product Gene Name   

EPAS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603349
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 Species Reactivity    Human
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 Specificity    No significant cross-reactivity or interference between this analyte and analogues is observed.
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 Samples    Body fluids, tissue homogenates, secretions or feces samples
 Assay Type    Quantitative Sandwich
 Detection Range    3.12pg/ml-100pg/ml
 Sensitivity    1.0pg/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EPAS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EPAS1 purchase    MBS9361593 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endothelial PAS Domain-Containing Protein 1 (EPAS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12pg/ml-100pg/ml in biological research samples containing EPAS1, with an estimated sensitivity of 1.0pg/ml. The ELISA analytical biochemical technique of the MBS9361593 kit is based on EPAS1 antibody-EPAS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EPAS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EPAS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Body fluids, tissue homogenates, secretions or feces samples. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for EPAS1 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of EPAS1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9361593. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EPAS1. It may not necessarily be applicable to this product.
 NCBI GI #    40254439
 NCBI GeneID    2034
 NCBI Accession #    NP_001421.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001430.4 [Other Products]
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 UniProt Secondary Accession #    Q86VA2; Q99630 [Other Products]
 UniProt Related Accession #    Q99814 [Other Products]
 Molecular Weight    96,459 Da
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 NCBI Official Full Name    endothelial PAS domain-containing protein 1
 NCBI Official Synonym Full Names    endothelial PAS domain protein 1
 NCBI Official Symbol    EPAS1 [Similar Products]
 NCBI Official Synonym Symbols   
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
[Similar Products]
 NCBI Protein Information    endothelial PAS domain-containing protein 1
 UniProt Protein Name    Endothelial PAS domain-containing protein 1
 UniProt Synonym Protein Names   
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
 Protein Family    Endothelial PAS domain-containing protein
 UniProt Gene Name    EPAS1 [Similar Products]
 UniProt Synonym Gene Names    BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha [Similar Products]
 UniProt Entry Name    EPAS1_HUMAN
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 NCBI Summary for EPAS1    This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
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 UniProt Comments for EPAS1    HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2p21-p16

Cellular Component: cytosol; nuclear speck; nucleoplasm; transcription factor complex

Molecular Function: DNA binding; histone acetyltransferase binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding

Biological Process: angiogenesis; blood vessel remodeling; cell maturation; embryonic placenta development; erythrocyte differentiation; iron ion homeostasis; lung development; mitochondrion organization and biogenesis; myoblast cell fate commitment; norepinephrine metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of heart rate; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to hypoxia; signal transduction; somatic stem cell maintenance; surfactant homeostasis; transcription from RNA polymerase II promoter; visual perception

Disease: Erythrocytosis, Familial, 4
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 Research Articles on EPAS1    1. HIF3alpha has a transcriptional regulatory function, which negatively affects gene expression by competing with HIF1alpha and HIF2alpha in binding to transcriptional elements in target genes during hypoxia. (Review)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with EPAS1 elisa kitPathways associated with EPAS1 elisa kit
 Reference Product  PubMed Publications
 EGLN1 elisa kit  >26 publications with EPAS1 and EGLN1
 VHL elisa kit  >24 publications with EPAS1 and VHL
 ARNT elisa kit  >13 publications with EPAS1 and ARNT
 EPO elisa kit  >6 publications with EPAS1 and EPO
 FLT1 elisa kit  >5 publications with EPAS1 and FLT1
 ARNT2 elisa kit  >2 publications with EPAS1 and ARNT2
 EGLN2 elisa kit  >1 publications with EPAS1 and EGLN2
 EGLN3 elisa kit  >1 publications with EPAS1 and EGLN3
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway antibodies  Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
Diseases associated with EPAS1 elisa kitOrgans/Tissues associated with EPAS1 elisa kit
 Disease Name  Pubmed Publications
 Carcinoma, Renal Cell Antibodies  >13 publications with EPAS1 and Carcinoma, Renal Cell
 Disease Models, Animal Antibodies  >11 publications with EPAS1 and Disease Models, Animal
 Kidney Diseases Antibodies  >9 publications with EPAS1 and Kidney Diseases
 Lung Diseases Antibodies  >7 publications with EPAS1 and Lung Diseases
 Inflammation Antibodies  >6 publications with EPAS1 and Inflammation
 Necrosis Antibodies  >6 publications with EPAS1 and Necrosis
 Hypertrophy Antibodies  >5 publications with EPAS1 and Hypertrophy
 Breast Neoplasms Antibodies  >4 publications with EPAS1 and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >4 publications with EPAS1 and Carcinoma, Hepatocellular
 Hypertension Antibodies  >4 publications with EPAS1 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >44 publications with EPAS1 and Vascular
 Blood Antibodies  >43 publications with EPAS1 and Blood
 Lung Antibodies  >17 publications with EPAS1 and Lung
 Kidney Antibodies  >17 publications with EPAS1 and Kidney
 Heart Antibodies  >16 publications with EPAS1 and Heart
 Liver Antibodies  >14 publications with EPAS1 and Liver
 Muscle Antibodies  >11 publications with EPAS1 and Muscle
 Embryonic Tissue Antibodies  >10 publications with EPAS1 and Embryonic Tissue
 Brain Antibodies  >9 publications with EPAS1 and Brain
 Connective Tissue Antibodies  >8 publications with EPAS1 and Connective Tissue
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