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ET-1 elisa kit :: Human Endothelin 1 (ET-1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS261099
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Endothelin 1 (ET-1), ELISA Kit

★Popular Item★
 Also Known As   

Human Endothelin 1 (ET-1) ELISA Kit

 Product Gene Name   

ET-1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    131240
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    human serum, plasma or cell culture supernatant
 Detection Range    500 pg/ml-7.8 pg/ml
 Sensitivity    2 pg/mL
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ET-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ET-1 purchase    MBS261099 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endothelin 1 (ET-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 500 pg/ml-7.8 pg/ml in biological research samples containing ET-1, with an estimated sensitivity of 2 pg/mL. The ELISA analytical biochemical technique of the MBS261099 kit is based on ET-1 antibody-ET-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ET-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ET-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as human serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ET-1 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ET-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ET-1 elisa kit    ET-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS261099. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ET-1. It may not necessarily be applicable to this product.
 NCBI GI #    556203
 NCBI GeneID    1906
 NCBI Accession #    AAA52339.1 [Other Products]
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 UniProt Secondary Accession #    Q96DA1 [Other Products]
 UniProt Related Accession #    P05305 [Other Products]
 Molecular Weight    24,425 Da
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 NCBI Official Full Name    endothelin-1
 NCBI Official Synonym Full Names    endothelin 1
 NCBI Official Symbol    EDN1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    endothelin-1; preproendothelin-1
 UniProt Protein Name    Endothelin-1
 UniProt Synonym Protein Names   
Preproendothelin-1; PPET1
 UniProt Gene Name    EDN1 [Similar Products]
 UniProt Synonym Gene Names    PPET1; ET-1 [Similar Products]
 UniProt Entry Name    EDN1_HUMAN
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 NCBI Summary for ET-1    The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
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 UniProt Comments for ET-1    EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; prostaglandin biosynthetic process; sensory perception of pain; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; peptide hormone secretion; response to amino acid stimulus; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; G-protein signaling, phospholipase D activating pathway; positive regulation of cell size; neural crest cell development; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; negative regulation of cellular protein metabolic process; glucose transport; negative regulation of transcription from RNA polymerase II promoter; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; calcium-mediated signaling; positive regulation of hormone secretion; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; cartilage development; response to ozone; maternal process involved in parturition; positive regulation of prostaglandin secretion; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3
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 Research Articles on ET-1    1. ET1 expression (both protein and mRNA) was increased in reflux esophagitis and Barrett's esophagus compared to controls. In Barrett's, ET1 expression was higher in proximal than distal segments.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ET-1 elisa kitPathways associated with ET-1 elisa kit
 Reference Product  PubMed Publications
 EDNRA elisa kit  >35 publications with ET-1 and EDNRA
 EDNRB elisa kit  >25 publications with ET-1 and EDNRB
 JUN elisa kit  >19 publications with ET-1 and JUN
 ECE1 elisa kit  >14 publications with ET-1 and ECE1
 FOS elisa kit  >2 publications with ET-1 and FOS
 HIF1A elisa kit  >1 publications with ET-1 and HIF1A
 GATA2 elisa kit  >1 publications with ET-1 and GATA2
 AKT1 elisa kit  >1 publications with ET-1 and AKT1
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 EGFR-dependent Endothelin Signaling Events Pathway antibodies  EGFR-dependent Endothelin Signaling Events Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 Endothelins Pathway antibodies  Endothelins Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
Diseases associated with ET-1 elisa kitOrgans/Tissues associated with ET-1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >31 publications with ET-1 and Cardiovascular Diseases
 Hypertension Antibodies  >26 publications with ET-1 and Hypertension
 Craniofacial Abnormalities Antibodies  >16 publications with ET-1 and Craniofacial Abnormalities
 Inflammation Antibodies  >15 publications with ET-1 and Inflammation
 Asthma Antibodies  >13 publications with ET-1 and Asthma
 Cardiovascular Abnormalities Antibodies  >6 publications with ET-1 and Cardiovascular Abnormalities
 Kidney Diseases Antibodies  >6 publications with ET-1 and Kidney Diseases
 Necrosis Antibodies  >5 publications with ET-1 and Necrosis
 Heart Defects, Congenital Antibodies  >5 publications with ET-1 and Heart Defects, Congenital
 Heart Failure Antibodies  >4 publications with ET-1 and Heart Failure
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with ET-1 and Brain
 Liver Antibodies  >3 publications with ET-1 and Liver
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