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EN2 elisa kit :: Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024118 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Engrailed Homeobox Protein 2 (EN2), ELISA Kit

★Popular Item★
 Also Known As   

Engrailed Homeobox Protein 2 (EN2) ELISA Kit

 Product Synonym Names    AUTS1
 Product Gene Name   

EN2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P02836
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Engrailed Homeobox Protein 2 (EN2).
No significant cross-reactivity or interference between Engrailed Homeobox Protein 2 (EN2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.113ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Engrailed Homeobox Protein 2 (EN2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Engrailed Homeobox Protein 2 (EN2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in an a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EN2 purchase    MBS2024118 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Engrailed Homeobox Protein 2 (EN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing EN2, with an estimated sensitivity of < 0.113ng/mL. The ELISA analytical biochemical technique of the MBS2024118 kit is based on EN2 antibody-EN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EN2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Engrailed Homeobox Protein 2 (EN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Engrailed Homeobox Protein 2 (EN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Engrailed Homeobox Protein 2 (EN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Engrailed Homeobox Protein 2 (EN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for EN2 elisa kit    Tumor immunity
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 Typical Standard Curve/Testing Data of EN2 elisa kit    EN2 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2024118. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EN2. It may not necessarily be applicable to this product.
 NCBI GI #    24652772
 NCBI GeneID    36240
 NCBI Accession #    NP_725059.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_165841.2 [Other Products]
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 UniProt Primary Accession #    P02836 [Other Products]
 UniProt Secondary Accession #    P02837; Q0E9C0; Q24356; Q9V601 [Other Products]
 UniProt Related Accession #    P02836 [Other Products]
 Molecular Weight    59,411 Da
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 NCBI Official Full Name    engrailed, isoform B
 NCBI Official Synonym Full Names    engrailed
 NCBI Official Symbol    en [Similar Products]
 NCBI Official Synonym Symbols   
153867_at; Apa; CG9015; DmelCG9015; En; EN; en1; Eng; Engr; Engrailed; Engrailed/Invected; EnR; Es; spa2; spt; V
[Similar Products]
 NCBI Protein Information    CG9015 gene product from transcript CG9015-RB
 UniProt Protein Name    Segmentation polarity homeobox protein engrailed
 Protein Family    Homeobox protein
 UniProt Gene Name    en [Similar Products]
 UniProt Entry Name    HMEN_DROME
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 UniProt Comments for EN2    This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
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 Research Articles on EN2    1. Data indicate there is redundancy in both regulatory DNA and protein function in invected (inv) and engrailed (en) genes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with EN2 elisa kitOrgans/Tissues associated with EN2 elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >11554 publications with EN2 and Kidney Diseases
 Poisoning Antibodies  >8052 publications with EN2 and Poisoning
 Paralysis Antibodies  >1046 publications with EN2 and Paralysis
 Acute Kidney Injury Antibodies  >997 publications with EN2 and Acute Kidney Injury
 Hypotension, Orthostatic Antibodies  >50 publications with EN2 and Hypotension, Orthostatic
 Inappropriate ADH Syndrome Antibodies  >10 publications with EN2 and Inappropriate ADH Syndrome
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >8181 publications with EN2 and Testis
 Head Antibodies  >7876 publications with EN2 and Head
 Whole Body Antibodies  >2232 publications with EN2 and Whole Body
 Uncharacterized Tissue Antibodies  >175 publications with EN2 and Uncharacterized Tissue
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