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ERalpha elisa kit :: Human Estrogen Receptor Alpha (ERalpha) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2600479
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $260 +1 FREE 8GB USB
96-Strip-Wells  /  $405 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
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 Product Name   

Estrogen Receptor Alpha (ERalpha), ELISA Kit

★Popular Item★
 Also Known As   

Human Estrogen Receptor Alpha (ERalpha) ELISA Kit

 Product Synonym Names    Human Estrogen Receptor Alpha (ERa) ELISA Kit
 Product Gene Name   

ERalpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 615363
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ERalpha concentration
 Assay Type    Sandwich
 Detection Range    40 ng/ml-0.625 ng/ml
 Sensitivity    0.1ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ERalpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ERalpha purchase    MBS2600479 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estrogen Receptor Alpha (ERalpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 40 ng/ml-0.625 ng/ml in biological research samples containing ERalpha, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS2600479 kit is based on ERalpha antibody-ERalpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERalpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERalpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ERalpha concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ERalpha elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ERalpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of ERalpha elisa kit    ERalpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2600479. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ERalpha. It may not necessarily be applicable to this product.
 NCBI GI #    5821726
 NCBI Accession #    AAD52984.1 [Other Products]
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 UniProt Secondary Accession #    Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7 [Other Products]
 Molecular Weight    35,547 Da
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 NCBI Official Full Name    estrogen receptor alpha, partial
 UniProt Protein Name    Estrogen receptor
 UniProt Synonym Protein Names   
ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
 UniProt Gene Name    ESR1 [Similar Products]
 UniProt Synonym Gene Names    ESR; NR3A1; ER [Similar Products]
 UniProt Entry Name    ESR1_HUMAN
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 UniProt Comments for ERalpha    ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; antral ovarian follicle growth; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gene expression; positive regulation of transcription factor activity

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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