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FAS Ligand elisa kit :: Human FAS Ligand ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355284
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $465 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FAS Ligand, ELISA Kit

★Popular Item★
 Also Known As   

FAS Ligand Human ELISA Kit

 Product Synonym Names    Human FAS Ligand
 Product Gene Name   

FAS Ligand elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS355284 QC Report    QC Report PDF
 Species Reactivity    Human
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 Assay Type    Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml
 Sensitivity    < 2 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FAS Ligand elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FAS Ligand purchase    MBS355284 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FAS Ligand, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing FAS Ligand, with an estimated sensitivity of < 2 pg/ml. The ELISA analytical biochemical technique of the MBS355284 kit is based on FAS Ligand antibody-FAS Ligand antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAS Ligand antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAS Ligand. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FAS Ligand elisa kit

   Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FASL polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FASL polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FASL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FASL can be calculated.

Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. It may be instrumental in the prevention of leukocyte trafficking between the mother and the fetus, although no pregnancy defects have yet been attributed to a faulty Fas-Fas ligand system.
 Applications Tested/Suitable for FAS Ligand elisa kit   

Quantitative sELISA (EIA)

 Application Notes for FAS Ligand elisa kit    For quantitative detection of FASL in Human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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 Typical Testing Data/Standard Curve (for reference only) of FAS Ligand elisa kit    FAS Ligand elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355284. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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