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FGF2 elisa kit :: Human FGF2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS8244557
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
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 Product Name   

FGF2, ELISA Kit

★Popular Item★
 Also Known As   

Human FGF2 ELISA Kit

 Product Synonym Names    FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
 Product Gene Name   

FGF2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    134920
 3D Structure    ModBase 3D Structure for P09038
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 Species Reactivity    Human
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 Specificity    The Human FGF2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human FGF2 proteins within the range of 7.8 pg/ml - 500 pg/ml.
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 Sensitivity    <4 pg/mL
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FGF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FGF2 purchase    MBS8244557 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FGF2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGF2, with an estimated sensitivity of <4 pg/mL. The ELISA analytical biochemical technique of the MBS8244557 kit is based on FGF2 antibody-FGF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FGF2 elisa kit

   Principle of the Assay: MBS Human FGF2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF2 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human FGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Human FGF-Basic or Fibroblast Growth Factor 2, also known commonly as HBGF-2 or Heparin-Binding Growth Factor 2, is a 288 amino acid cytokine protein encoded by the FGF2gene at locus 4q26 on chromosome 4. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, would healing and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The monomeric FGF-Basic protein interacts with many other proteins such as CSPG4 and FGFBP1 while also believed to be found in a complex with FGFBP1, FGF1 and FGF2. Expression patterns for this protein typically take place in granulosa and cumulus cells, but FGF2 is also found in hepatocellular carcinoma cells (not non-cancerous liver tissue). Several experiments were performed on FGF-Basic to determine its pH and temperature properties. It has been recently determined that FGF-Basic retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20C, while being inactivated after 3 minutes at 60C or 1 minute at 80C.
 Applications Tested/Suitable for FGF2 elisa kit   

Sandwich ELISA (EIA)

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 Typical Testing Data/Standard Curve (for reference only) of FGF2 elisa kit    FGF2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS8244557. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FGF2. It may not necessarily be applicable to this product.
 NCBI GI #    261260095
 NCBI GeneID    2247
 NCBI Accession #    P09038.3 [Other Products]
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 UniProt Primary Accession #    P09038 [Other Products]
 UniProt Secondary Accession #    O00527; P78443; Q16443; Q5PY50; Q7KZ11; Q7KZ72; Q9UC54; Q9UCS5; Q9UCS6; A4LBB8 [Other Products]
 UniProt Related Accession #    P09038 [Other Products]
 Molecular Weight    21,203 Da
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 NCBI Official Full Name    Fibroblast growth factor 2
 NCBI Official Synonym Full Names    fibroblast growth factor 2
 NCBI Official Symbol    FGF2 [Similar Products]
 NCBI Official Synonym Symbols   
BFGF; FGFB; FGF-2; HBGF-2
[Similar Products]
 NCBI Protein Information    fibroblast growth factor 2
 UniProt Protein Name    Fibroblast growth factor 2
 UniProt Synonym Protein Names   
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
 Protein Family    Fibroblast growth factor
 UniProt Gene Name    FGF2 [Similar Products]
 UniProt Synonym Gene Names    FGFB; FGF-2; bFGF; HBGF-2 [Similar Products]
 UniProt Entry Name    FGF2_HUMAN
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 NCBI Summary for FGF2    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FGF2    FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: extracellular region; extracellular space; nucleus

Molecular Function: chemoattractant activity; cytokine activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; cell migration during sprouting angiogenesis; chemotaxis; embryonic morphogenesis; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; growth factor dependent regulation of satellite cell proliferation; hyaluronan catabolic process; innate immune response; inositol phosphate biosynthetic process; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of blood vessel endothelial cell migration; nerve growth factor receptor signaling pathway; nervous system development; organ morphogenesis; phosphatidylinositol biosynthetic process; phosphoinositide-mediated signaling; positive chemotaxis; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cardiac muscle cell proliferation; positive regulation of cell division; positive regulation of cell fate specification; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of MAP kinase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; Ras protein signal transduction; regulation of angiogenesis; release of sequestered calcium ion into cytosol; signal transduction; small GTPase mediated signal transduction; somatic stem cell maintenance; ureteric bud branching; vascular endothelial growth factor receptor signaling pathway; wound healing
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 Research Articles on FGF2    1. these multifaceted effects of FGF-2 promote new tissue formation at the early regeneration phase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FGF2 elisa kitPathways associated with FGF2 elisa kit
 Reference Product  PubMed Publications
 FGFR1 elisa kit  >702 publications with FGF2 and FGFR1
 FGFR2 elisa kit  >385 publications with FGF2 and FGFR2
 FGFR3 elisa kit  >150 publications with FGF2 and FGFR3
 FGFR4 elisa kit  >72 publications with FGF2 and FGFR4
 NANOG elisa kit  >70 publications with FGF2 and NANOG
 SDC1 elisa kit  >54 publications with FGF2 and SDC1
 SDC4 elisa kit  >34 publications with FGF2 and SDC4
 SDC2 elisa kit  >12 publications with FGF2 and SDC2
 HSPG2 elisa kit  >7 publications with FGF2 and HSPG2
 GPC1 elisa kit  >4 publications with FGF2 and GPC1
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activated Point Mutants Of FGFR2 Pathway antibodies  Activated Point Mutants Of FGFR2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
Diseases associated with FGF2 elisa kitOrgans/Tissues associated with FGF2 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1084 publications with FGF2 and Nervous System Diseases
 Necrosis Antibodies  >639 publications with FGF2 and Necrosis
 Wounds and Injuries Antibodies  >611 publications with FGF2 and Wounds and Injuries
 Inflammation Antibodies  >480 publications with FGF2 and Inflammation
 Heart Diseases Antibodies  >435 publications with FGF2 and Heart Diseases
 Disease Progression Antibodies  >359 publications with FGF2 and Disease Progression
 Myocardial Ischemia Antibodies  >357 publications with FGF2 and Myocardial Ischemia
 Lung Diseases Antibodies  >351 publications with FGF2 and Lung Diseases
 Hyperplasia Antibodies  >264 publications with FGF2 and Hyperplasia
 Lung Neoplasms Antibodies  >238 publications with FGF2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >4886 publications with FGF2 and Vascular
 Brain Antibodies  >2256 publications with FGF2 and Brain
 Bone Antibodies  >1960 publications with FGF2 and Bone
 Muscle Antibodies  >1853 publications with FGF2 and Muscle
 Embryonic Tissue Antibodies  >1308 publications with FGF2 and Embryonic Tissue
 Eye Antibodies  >1049 publications with FGF2 and Eye
 Skin Antibodies  >853 publications with FGF2 and Skin
 Connective Tissue Antibodies  >763 publications with FGF2 and Connective Tissue
 Bone Marrow Antibodies  >702 publications with FGF2 and Bone Marrow
 Heart Antibodies  >685 publications with FGF2 and Heart
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